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HelpOrder Code LAB6080 Bacterial Culture, Anaerobic, Varies
Additional Codes
Mayo Order Code: ANAE
EPIC Test Code: LAB6080
Reporting Name
Bacterial Culture, AnaerobicUseful For
Diagnosing anaerobic bacterial infections
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesShipping Instructions
Specimen should arrive within 72 hours of collection.
Necessary Information
Specimen source is required.
Specimen Required
Supplies: Anaerobe Transport Tube (T588)
Specimen Types: Deep tissues, sterile body fluids, abscesses, percutaneous transtracheal aspirates, suprapubic aspirations, or wounds
Collection Instructions: Specimen should be obtained by using a needle and syringe from a source not normally colonized by anaerobes.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient | 72 hours |
Reference Values
No growth
Identification of probable pathogens
Day(s) Performed
Monday through Sunday
CPT Code Information
87075-Bacterial Culture, Anaerobic
87076-Id MALDI-TOF Mass Spec Anaerobe (if appropriate)
87153-Anaerobe Ident by Sequencing (if appropriate)
87176-Tissue Processing (if appropriate)
87150-Identification by PCR (if appropriate)
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RMALA | Id MALDI-TOF Mass Spec Anaerobe | No, (Bill Only) | No |
| ISAN | Anaerobe Ident by Sequencing | No, (Bill Only) | No |
| TISSR | Tissue Processing | No, (Bill Only) | No |
| ANAID | Anaerobe Ident | No, (Bill Only) | No |
| PCRID | Identification by PCR | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex tests may be performed at an additional charge.
Method Description
Appropriate specimens are inoculated onto blood agar, phenylethyl alcohol agar, lysed blood agar containing gentamicin and vancomycin, and into thioglycollate broth tubes which are incubated anaerobically for 48 hours. Colonies are subcultured to determine aerotolerance, and obligately anaerobic organisms may be identified by Gram stain and/or use of various differential media, conventional biochemical tests, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, real-time polymerase chain reaction (RT PCR), or 16S ribosomal RNA gene sequencing (rRNA).(Procop GW, Church DL, Hall GS, et al. eds: The anaerobic bacteria. In: Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 7th ed. Wolters Kluwer Lippincott Williams and Wilkins; 2017:983-1073)
Reject Due To
| Swab | Reject |
Method Name
Conventional Culture Technique