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Order Code LAB614 Ascorbic Acid (Vitamin C), Plasma

Important Note

MAY ONLY BE COLLECTED AT RMC HOSPITAL LAB

  1. Place carrier from DASH centrifuge in -70 freezer for 10 min. prior to spinning specimen
  2. Draw specimen in a cooled dark green tube and place immediately on wet ice
  3. Spin in DASH centrifuge and remove immediately
  4. Separate plasma to an AMBER VIAL and place in the -70 degre freezer in Chemistry until completely frozen.  Specimen may be placed in the Mayo freezer once solidly frozen.

 

Additional Codes

Mayo Code VITC

EPIC Test Code: LAB614

Useful For

Identifying vitamin C deficiency

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Portions of this test are covered by patents held by Quest Diagnostics

Reporting Name

Ascorbic Acid, P

Specimen Type

Plasma Heparin


Shipping Instructions


Ship specimen frozen on dry ice in amber vial to protect from light.



Specimen Required


Patient Preparation: Fasting overnight (12-14 hours) (infants-collect prior to next feeding). Water can be taken as needed.

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube:

Preferred: Green top (sodium or lithium heparin)

Submission Container/Tube: Amber vial

Specimen Volume: 1 mL

Collection Instructions:

1. Immediately place specimen on wet ice and process within 4 hours of collection.

2. Centrifuge at 4° C, aliquot plasma into amber vial to protect from light.

3. Freeze plasma immediately, ideally at or below -60° C, protected from light.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Heparin Frozen 14 days LIGHT PROTECTED

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Reference Values

0.4-2.0 mg/dL

Method Description

Samples are diluted and extracted online extraction by high-turbulence liquid chromatography, with detection by tandem mass spectrometry.(Unpublished Mayo Method)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

82180

Forms

If not ordering electronically, complete, print, and send General Request (T239) with the specimen.