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Order Code LABAACDQ Amino Acids, Quantitative, Random, Urine

Additional Codes

Mayo Test Code: AAPD
EPIC Order Code: LABAACDQ

Reporting Name

Amino Acids, QN, Random, U

Useful For

Evaluating patients with possible inborn errors of metabolism using random urine specimens

 

May aid in evaluation of endocrine disorders, liver diseases, muscle diseases, neoplastic diseases, neurological disorders, nutritional disturbances, kidney failure, and burns

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Urine


Additional Testing Requirements


Not all patients with homocystinuria will be detected by this assay. If homocystinuria is a concern, order CMMPP / Cobalamin, Methionine, and Methylmalonic Acid Pathways, Plasma or HCYSP / Homocysteine, Total, Plasma or HCYSS / Homocysteine, Total, Serum in tandem with this test.



Necessary Information


1. Patient's age is required.

2. Include family history, clinical condition (asymptomatic or acute episode), diet, and drug therapy information.

3. If prolidase deficiency is a concern, indicate on the amino acid order "Pretreat with acid hydrolysis prior to analysis". The acid hydrolysis will break up in vitro proline and hydroxyproline containing dipeptides, which are cleaved in vivo by prolidase.



Specimen Required


Supplies: Urine Tubes, 10 mL (T068)

Specimen Volume: 2 mL

Collection Instructions: Collect a random urine specimen.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Frozen (preferred) 70 days
  Refrigerated  14 days

Reference Values

Amino Acid

Age groups

< 2 months

2-35 months

3-6 years

7-17 years

≥18 years

Taurine

Tau

<9026

<5604

<3680

<3954

<2134

Asparagine

Asn

<687

<1159

<238

<322

<204

Serine

Ser

<3202

<2741

<1386

<1116

<658

Hydroxyproline

Hyp

<2254

<1775

<89

<42

<48

Glycine

Gly

<13336

<9872

<4997

<4467

<4068

Glutamine

Gln

<2931

<3681

<1124

<1038

<764

Aspartic Acid

Asp

<290

<72

<46

<13

<12

Ethanolamine

EtN

<1589

<1765

<776

<536

<500

Histidine

His

<3806

<4578

<2596

<2294

<1508

Threonine

Thr

<1930

<1769

<496

<575

<384

Citrulline

Cit

<61

<67

<23

<17

<34

Sarcosine

Sar

<177

<52

<6

<10

<6

Beta-Alanine

bAla

<174

<79

<99

<139

<58

Alanine

Ala

<2856

<2360

<961

<794

<472

Glutamic Acid

Glu

<281

<210

<127

<63

<62

1-Methylhistidine

1MHis

<493

<470

<517

<453

<265

3-Methylhistidine

3MHis

<196

<662

<3346

<2027

<1920

Argininosuccinic Acid

Asa

<96

<103

<65

<57

<37

Homocitrulline

Hcit

<164

<169

<110

<107

<87

Arginine

Arg

<250

<147

<81

<42

<65

Alpha-aminoadipic Acid

Aad

<264

<397

<349

<138

<73

Gamma Amino-n-butyric Acid

GABA

<8

<14

<4

<3

<4

Beta-aminoisobutyric Acid

bAib

<2214

<2488

<840

<251

<265

Alpha-amino-n-butyric Acid

Abu

<58

<44

<37

<29

<19

Hydroxylysine

Hyl

<101

<87

<44

<22

<16

Proline

Pro

<1776

<1028

<29

<42

<98

Ornithine

Orn

<344

<238

<228

<291

<193

Cystathionine

Cth

<118

<53

<23

<24

<33

Cystine

Cys

<486

<285

<111

<84

<142

Lysine

Lys

<2217

<1321

<814

<463

<295

Methionine

Met

<76

<45

<24

<22

<15

Valine

Val

<308

<258

<119

<91

<73

Tyrosine

Tyr

<453

<603

<289

<371

<155

Isoleucine

Ile

<99

<131

<52

<29

<26

Leucine

Leu

<286

<215

<87

<70

<64

Phenylalanine

Phe

<342

<306

<178

<123

<98

Tryptophan

Trp

<241

<329

<222

<218

<140

Allo-isoleucine

AlloIle

<3

<6

<1

<2

<4

 

All results reported as nmol/mg creatinine.

Day(s) Performed

Monday through Friday

CPT Code Information

82139

Method Description

Quantitative analysis of amino acids is performed by liquid chromatography-tandem mass spectrometry. Patient samples are combined with isotopically labeled internal standard. Following protein precipitation, the supernatant is subjected to hydrophilic-interaction liquid chromatography for the separation of isomers with MS/MS detection of the underivatized amino acids.(Unpublished Mayo method)

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Testing Algorithm

Testing includes quantitation of the following amino acids: taurine, threonine, serine, asparagine, hydroxyproline, glutamic acid, glutamine, aspartic acid, ethanolamine, sarcosine, proline, glycine, alanine, citrulline, alpha-aminoadipic acid, alpha-amino-n-butyric acid, valine, cystine, cystathionine, methionine, isoleucine, leucine, tyrosine, phenylalanine, beta-alanine, beta-aminoisobutyric acid, ornithine, lysine, 1-methylhistidine, histidine, 3-methylhistidine, argininosuccinic acid, allo-isoleucine, homocitrulline, gamma-amino-n-butyric acid, hydroxylysine, tryptophan, and arginine.

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Portions of this test are covered by patents held by Quest Diagnostics