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HelpOrder Code LABAFPPT Alpha-Fetoprotein (AFP), Peritoneal Fluid
Additional Codes
Mayo Test Code: AFPPT
Reporting Name
AFP, Peritoneal FluidUseful For
An adjunct to cytology to differentiate between malignancy-related ascites and benign causes of ascites formation
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
PeritonealSpecimen Required
Sources: Peritoneal, abdominal, ascites, paracentesis (peritoneal washings are not acceptable)
Container/Tube: Plain, plastic, screw-top tube
Specimen Volume: 2 mL
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Peritoneal | Frozen (preferred) | 90 days |
| Ambient | 7 days | |
| Refrigerated | 7 days |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Saturday
CPT Code Information
86316
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| AFPPT | AFP, Peritoneal Fluid | 49761-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| AFPPN | AFP, Peritoneal Fluid | 49761-0 |
| SITEF | Site | 39111-0 |
Method Description
The instrument used is the Beckman Coulter UniCel DxI 800. The Beckman Coulter Access alpha-fetoprotein (AFP) immunoassay is a 2-site immunoenzymatic sandwich assay. A specimen is added to a reaction vessel with mouse monoclonal anti-AFP alkaline phosphatase conjugate, and paramagnetic particles coated with a second mouse monoclonal anti-AFP antibody. The AFP in the specimen binds to the immobilized monoclonal anti-AFP on the solid phase while, at the same time, the monoclonal anti-AFP-alkaline phosphatase conjugate reacts with different antigenic sites on the specimen AFP. After incubation in a reaction vessel, materials bound by the solid phase are held in a magnetic field while unbound materials are washed away. A chemiluminescent substrate is added to the reaction vessel, and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the amount of AFP in the specimen. The amount of analyte in the specimen is determined by means of a stored multipoint calibration curve.(Package insert: Access AFP assay, Beckman Coulter Inc; 2024)
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Immunoenzymatic Assay
Forms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.