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HelpOrder Code LABAMBF Amylase, Body Fluid
Reporting Name
Amylase, BFUseful For
Evaluation of patients with a pathological accumulation of fluid to determine whether pancreatic inflammation, pancreatic fistula, or esophageal rupture may be contributing
Aiding in the diagnosis of pancreatitis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Body FluidOrdering Guidance
For pancreatic cyst fluid specimens, order AMLPC / Amylase, Pancreatic Cyst Fluid. Testing will be changed to AMLPC if this test is ordered on that specimen type.
Necessary Information
1. Date and time of collection are required.
2. Specimen source is required.
Specimen Required
Specimen Type: Body fluid
Preferred Source:
-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)
-Pleural fluid (pleural, chest, thoracentesis)
-Drain fluid (drainage, Jackson Pratt [JP] drain)
-Pericardial fluid
Acceptable Source: Write in source name with source location (if appropriate)
Collection Container/Tube: Sterile container
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge to remove any cellular material and transfer into a plastic vial.
2. Indicate the specimen source and source location on label.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Body Fluid | Refrigerated (preferred) | 7 days | |
| Frozen | 30 days | ||
| Ambient | 24 hours |
Reference Values
An interpretive report will be provided
Day(s) Performed
Monday through Sunday
CPT Code Information
82150
Method Description
The liquid Roche amylase method is an enzymatic colorimetric test using 4,6-ethyliden (G7)-p-nitrophenol (G1)-alpha, D-maltoheptaoside (ethylidene-G7PNP) as a substrate. Human salivary and pancreatic amylases convert the substrate at approximately the same rate. The alpha-amylase cleaves the substrate into G2, G3, G4 PNP fragments. The G2 and G3 and G4 PNP fragments are further hydrolyzed by an alpha-glucosidase to yield p-nitrophenol and glucose. The rate of increase in absorbance at 415 nm (measuring the increase in p-nitrophenol) is proportional to amylase activity.(Package insert: Roche AMYL reagent. Roche Diagnostic Corp; V12.0, 02/2022)
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Anticoagulant or additive, breast milk, nasal secretions, gastric secretions, bronchoalveolar lavage (BAL or bronchial washings), feces, colostomy/ ostomy, saliva, sputum, urine, vitreous fluid, or pancreatic cyst | Reject |
Method Name
Enzymatic Colorimetric
Forms
If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request (T728) with the specimen.