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HelpOrder Code LABAMLPC Amylase, Pancreatic Cyst Fluid
Additional Codes
MAYO TEST CODE: AMLPC
Reporting Name
Amylase, Pancreatic CystUseful For
Aiding in distinguishing between pseudocysts and other types of pancreatic cysts when used in conjunction with imaging studies, cytology, and other pancreatic cyst fluid tumor markers
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Pancreatic Cyst FluidOrdering Guidance
For other body fluid specimens (eg, peritoneal, pleural), order AMBF / Amylase, Body Fluid. Testing will be changed to AMBF if this test is ordered on any fluid other than pancreatic fluid.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Container/Tube: Plain vial
Specimen Volume: 1 mL
Additional Information: A minimum of 0.5 mL is required for testing; specimens less than 0.5 mL may be rejected.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Pancreatic Cyst Fluid | Frozen (preferred) | 30 days |
| Ambient | 7 days | |
| Refrigerated | 7 days |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Saturday
CPT Code Information
82150
Method Description
The Roche amylase method is an enzymatic colorimetric test using 4,6-ethylidene (G7)-p-nitrophenol (G1)-alpha, D-maltoheptaoside (ethylidene-G7PNP) as a substrate. Human salivary and pancreatic amylases (alpha-amylase) convert the substrate at approximately the same rate. The alpha-amylase cleaves the substrate into G2, G3, G4 p-nitrophenol (PNP) fragments. The G2, G3, and G4 PNP fragments are further hydrolyzed by an alpha-glucosidase to yield PNP and glucose. The rate of increase in absorbance at 415 nm (measuring the increase in PNP) is directly proportional to amylase activity.(Package insert: Roche AMYL2 reagent. Roche Diagnostic Corp; V10 12/2018)
Reject Due To
| Gross hemolysis | Reject |
Method Name
Substrate Kinetic
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Oncology Test Request (T729)