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HelpOrder Code LABAT3AG Antithrombin Antigen, Plasma
Additional Codes
Mayo Test Code: ATTI
Reporting Name
Antithrombin Antigen, PUseful For
Assessing abnormal results of the antithrombin activity assay (ATTF / Antithrombin Activity, Plasma), the recommended primary (screening) antithrombin assay
Diagnosing antithrombin deficiency, acquired or congenital, in conjunction with measurement of antithrombin activity
An adjunct in the diagnosis and management of carbohydrate-deficient glycoprotein syndromes
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Plasma Na CitOrdering Guidance
For monitoring treatment of antithrombin deficiency disorders, including infusion of antithrombin therapeutic concentrate, order ATTF / Antithrombin Activity, Plasma.
Necessary Information
If patient is being treated with heparin, this should be noted as heparin treatment may lower plasma antithrombin.
Specimen Required
Specimen Type: Platelet-poor plasma
Patient Preparation: Fasting preferred
Collection Container/Tube: Light-blue top (3.2% sodium citrate)
Submission Container/Tube: Polypropylene vial
Specimen Volume: 1 mL
Collection Instructions:
1. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.
2. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.
3. Aliquot plasma into a plastic vial leaving 0.25 mL in the bottom of centrifuged vial.
4. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally, at-40° C or below.
Additional Information:
1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. Each coagulation assay requested should have its own vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma Na Cit | Frozen | 14 days |
Special Instructions
Reference Values
Adults: 80-120%
Normal, full-term newborn infants may have decreased levels (≥35-40%) that reach adult levels by 180 days postnatal.*
Healthy, premature infants (30-36 weeks gestation) may have decreased levels that reach adult levels by 180 days postnatal.*
*See Pediatric Hemostasis References section in Coagulation Guidelines for Specimen Handling and Processing.
Day(s) Performed
Monday through Friday
CPT Code Information
85301
Method Description
This assay is performed on the Instrumentation Laboratory ACL TOP using the Diagnostica Stago LIATEST AT III kit. Antithrombin antigen is determined using automated latex immunoassay (LIA) methodology. Patient plasma, containing antithrombin antigen, is combined with a latex reagent containing rabbit antihuman antibodies. An antigen-antibody reaction takes place, causing the latex particles to agglutinate and form aggregates. The aggregates form diameters greater than the wavelength of the light (405 nm) passing through causing absorption of the light. This change in absorption is measured over time and reported as delta optical density. The increase in absorption is proportional to the concentration of antithrombin antigen present in the sample.(Package insert: LIATEST AT III. Diagnostica Stago S.A.S.; Rev. 02/2015)
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Method Name
Latex Immunoassay (LIA)
Forms
If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.