Home
HelpOrder Code LABBFBL Bilirubin, Body Fluid
Reporting Name
Bilirubin, BFUseful For
Evaluating peritoneal fluid or abdominal drain fluid as a screening test for bile leakage
May aid in the distinction between a transudative and an exudative pleural effusion
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Body FluidOrdering Guidance
For bilirubin testing on urine specimens, order BILUR / Bilirubin, Random, Urine. Testing will be changed to BILUR if this test is ordered on urine specimens.
Shipping Instructions
Ship specimen in amber vial to protect from light.
Necessary Information
1. Date and time of collection are required.
2. Specimen source is required.
Specimen Required
Supplies: Amber Frosted Tube, 5 mL (T915)
Preferred Source:
-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)
-Pleural fluid (pleural, chest, thoracentesis)
-Drain fluid (drainage, Jackson Pratt [JP] drain)
-Pericardial fluid
Acceptable Source: Other body fluid, write in source name with source location (if appropriate)
Collection Container/Tube: Sterile container
Submission Container/Tube: Opaque, amber vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge to remove any cellular material and transfer into an amber vial to protect from light.
2. Indicate the specimen source and source location on label.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Body Fluid | Frozen (preferred) | 70 days | LIGHT PROTECTED |
| Refrigerated | 14 days | LIGHT PROTECTED |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Sunday
CPT Code Information
82247
Method Description
Total bilirubin, in the presence of a suitable solubilizing agent, is coupled with 3,5-dichlorophenyl diazonium in a strongly acidic medium to produce azobilirubin. The intensity of the color of the azobilirubin produced is proportional to the total bilirubin concentration and is measured at 546/600 nm.(Package insert: Bilirubin Total Gen. 3. Roche Diagnostics; V9.0. 01/2020)
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Anticoagulant or additive Amniotic fluid Breast milk Saliva Sputum Cerebrospinal fluid Bronchoalveolar lavage (BAL) Bronchial washings Colostomy Ostomy Gastric secretions Nasal secretions Urine Feces Vitreous fluid Synovial fluid |
Reject |
Method Name
Photometric, Diazonium Salt