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HelpOrder Code LABBHYD Beta-Hydroxybutyrate, Serum
Reporting Name
Beta-Hydroxybutyrate, SUseful For
Monitoring therapy for diabetic ketoacidosis
Investigating the differential diagnosis of any patient presenting to the emergency room with hypoglycemia, acidosis, suspected alcohol ingestion, or an unexplained increase in the anion gap
In pediatric patients, the presence or absence of ketonemia/uria is an essential component in the differential diagnosis of inborn errors of metabolism
Serum beta-hydroxybutyrate is a key parameter monitored during controlled 24-hour fasts
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Serum gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial within 2 hours of collection.
Specimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 7 days | |
| Refrigerated | 7 days |
Reference Values
<0.4 mmol/L
Day(s) Performed
Monday through Sunday
CPT Code Information
82010
Method Description
D-3-hydroxybutyrate in the presence of NAD is converted to acetoacetate and NADH at pH 8.5 by D-3-hydroxybutyrate dehydrogenase. At this pH the reaction is favored to the right. The NADH is converted to a colored compound using INT and Diaphorase.(Package insert: Stanbio Beta-hydroxybutyrate LiquiColor Procedure No. 2440; DN: RBR.2440.00, 4/16/2002)
Reject Due To
| Gross hemolysis | Reject |
Method Name
Photometric, B-Hydroxybutyrate Dehydrogenase (B-HBDH)