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HelpOrder Code LABCATTH Cold Agglutinin Titer, Serum
Additional Codes
Mayo Test Code: CATTR
EPIC Order Code:LABCATTH
Ordering Guidance
The cold agglutinin test is not specific for Mycoplasma pneumoniae and is not recommended to diagnose M pneumoniae infections. To diagnose M pneumoniae infections, order MPRP / Mycoplasma (Mycoplasmoides) pneumoniae with Macrolide Resistance Reflex, Molecular Detection, PCR, Varies. For screening recent or past exposure to M pneumoniae, order MYCO / Mycoplasma pneumoniae Antibodies, IgG and IgM, Serum.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 4 mL
Pediatric Volume: 1 mL
Collections Instructions:
1. Use a warm pack to keep specimen at 37° C prior to and after collecting.
2. Allow specimen to clot at 37° C.
3. Centrifuge at 37° C and aliquot serum into plastic vial immediately after blood clots or within one hour of collection.
4. Do not refrigerate prior to separation of serum from red cells.
Useful For
Detection of cold agglutinins in patients with suspected cold agglutinin disease
This test is not recommended to diagnose Mycoplasma pneumoniae infections.
Method Name
Titration/Red Cell Agglutination
Reporting Name
Cold Agglutinin TiterSpecimen Type
Serum RedSpecimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 7 days |
| Frozen | 7 days | |
| Ambient | 72 hours |
Reject Due To
| Gross hemolysis | OK |
| Gross icterus | OK |
Reference Values
Titer results:
>64: Elevated
>1000: May be indicative of hemolytic anemia
Method Description
The titer is determined by making serial doubling dilutions of the patient's serum in 0.9% saline. Group O indicator red cells are added, and the serum-cell mixture is then incubated 60 to 120 minutes at 2 to 8° C. The titer end point range is determined by hemagglutination.(Cohn CS, Delaney M, Johnson ST, Katz LM, Schwartz J. eds: Technical Manual. 21st ed. AABB; 2023)
Day(s) Performed
Monday through Friday, Sunday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86157
Forms
If not ordering electronically, complete, print, and send an Benign Hematology Test Request (T755) with the specimen.