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Order Code LABCCRPT Reptilase Time, Plasma

Additional Codes

Mayo Test Code: RTSC


Specimen Required


Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. For complete instruction, see Coagulation Guidelines for Specimen Handling and Processing.

2. Centrifuge, remove plasma, and centrifuge plasma again.

3. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally below -40° C.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Useful For

Evaluation of a prolonged thrombin time (TT): It is mainly used to confirm or exclude the presence of heparin in the specimen or specimen type

 

Evaluating hypofibrinogenemia or dysfibrinogenemia in conjunction with the TT and fibrinogen assay

Method Name

Optical Clot-Based

Reporting Name

Reptilase Time, P

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

14.0-23.9 seconds

Method Description

The reptilase time assay is performed on the Instrumentation Laboratories ACL TOP. Patient plasma is combined with a reptilase reagent containing a thrombin-like enzyme triggering the coagulation process in the mixture. Time to clot formation is measured optically using a wavelength of 671 nm.(Owen CA, Bowie EJW, Thompson JH. Tests of hemostasis and blood coagulation. In: The Diagnosis of Bleeding Disorders. 2nd ed. Little, Brown and Company; 1975:85-154)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

85635

Forms

If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.