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HelpOrder Code LABCHLE Cholesteryl Esters, Serum
Additional Codes
Mayo Test Code: CHLE
Reporting Name
Cholesteryl Esters, SUseful For
Establishing a diagnosis of lecithin-cholesterol acyltransferase deficiency
Evaluating the extent of metabolic disturbance by bile stasis or liver disease
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial. Send refrigerated.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 7 days | |
| Frozen | 60 days | ||
| Ambient | 24 hours |
Reference Values
≥18 years: 60-80% of total cholesterol
Reference values have not been established for patients who are less than 18 years of age.
Day(s) Performed
Monday through Friday
CPT Code Information
84311
Method Description
Free cholesterol reacts with cholesterol oxidase to generate hydrogen peroxide which reacts with 3,5-dimethoxy-N-(2-hydroxy-3-sulfopropyl) aniline sodium (DAOS) and 4-aminoantipyrene to produce a blue pigment. The product is proportional to the serum free cholesterol concentration.(Fujifilm Free Cholesterol E. Fujifilm Wako Pure Chemical Corporation; 12/2021)
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | Reject |
Method Name
Enzymatic Colorimetric
Forms
If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the specimen.