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HelpOrder Code LABFBIOT Biotin, Serum
Ordering Guidance
If testing for biotinidase deficiency is requested, order BIOTS / Biotinidase, Serum.
Shipping Instructions
Freeze specimens within 3 days of collection and send frozen.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Measurement of biotin in serum
Assessment of biotin concentrations in individuals taking biotin supplements
Investigation of unexpected results from immunoassays that utilize biotin-streptavidin detection methods
This test is not useful as a screen for biotinidase deficiency.
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Biotin, SSpecimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 30 days | |
| Refrigerated | 7 days | ||
| Ambient | 24 hours |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
≥18 years: ≤0.3 ng/mL
Reference values have not been established for patients who are <18 years of age.
Method Description
Biotin (Vitamin B7) is quantified in serum by liquid chromatography tandem mass spectrometry.(Unpublished Mayo method)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84591
Day(s) Performed
Tuesday