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HelpOrder Code LABFBL Fungal Culture, Blood
Additional Codes
Mayo Test Code: BFBL
EPIC Test Code: LABFBL
Reporting Name
Fungal Culture, BloodUseful For
Diagnosis and treatment of the etiologic agents of fungemia
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole bloodSpecimen Required
Container/Tube:
Preferred: Green top (sodium or lithium heparin)
Acceptable: SPS
Specimen Volume: 4 mL
Pediatric Volume: 3 mL
Collection Instructions:
1. Send whole blood specimen in original tube. Do not aliquot.
2. SPS tubes are acceptable, but not preferred.
Note: SPS tubes must be clearly labeled as SPS. If label is obscured, sample may be cancelled, as ACD (also yellow top) is not an acceptable tube type.
Specimen Minimum Volume
Adults: 3 mL
Pediatrics: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole blood | Ambient (preferred) | 7 days | |
| Refrigerated | 7 days |
Reference Values
Negative
If positive, notification is made as soon as the positive culture is detected or identified.
Day(s) Performed
Monday through Sunday
CPT Code Information
87103-Blood
87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)
87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)
87107-Fungal identification panel A (if appropriate)
87107-Fungal identification panel B (if appropriate)
87150-Identification rapid PCR coccidioides (if appropriate)
87150 x 2- Identification Histoplasma/Blastomyces, PCR (if appropriate)
87153-D2 fungal sequencing identification (if appropriate)
87150- Id, Candida auris Rapid PCR (if appropriate)
Method Description
Blood is inoculated into a MycoF Lytic culture bottle (Becton Dickinson) bottle and continuously monitored on a blood culture instrument.
Identification of fungi is based on colonial and microscopic morphology, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, laboratory-developed real-time polymerase chain reaction assays and/or D2 rDNA gene sequencing, as applicable.(Babady NE, Buckwalter SP, Hall L, Le Febre KM, Binnicker MJ, Wengenack NL. Detection of Blastomyces dermatitidis and Histoplasma capsulatum from culture isolates and clinical specimens by use of real-time PCR. J Clin Microbiol. 2011;49[9]:3204-3208; Binnicker MJ, Buckwalter SP, Eisberner JJ, et al. Detection of Coccidioides species in clinical specimens by real-time PCR. J Clin Microbiol. 2007;45[1]:173-178; Dhiman N, Hall L, Wohlfiel SL, Buckwalter SP, Wengenack NL. Performance and cost analysis of matrix-assisted laser desorption ionization time of flight mass spectrometry for routine identification of yeast. J Clin Microbiol. 2011:49[4];1614-1616; Hall L, Wohlfiel SL, Roberts GD. Experience with the MicroSeq D2 large-subunit ribosomal DNA sequencing kit for identification of filamentous fungi encountered in the clinical laboratory. J Clin Microbiol. 2004;42[2]:622-626; Theel ES, Schmidt BH, Hall L, et al. Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2012;50[9]:3093-3095; Theel ES, Hall L, Mandrekar J, Wengenack NL. Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2011;49[12]:4067-4071)
Reject Due To
| Blood culture bottles (eg, BACTEC MycoF Lytic) Isolator Clotted |
Reject |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| D2F | D2 Fungal Sequencing Identification | No, (Bill Only) | No |
| FUNA | Fungal Ident Panel A | No, (Bill Only) | No |
| FUNB | Fungal Ident Panel B | No, (Bill Only) | No |
| LCCI | Ident Rapid PCR Coccidioides | No, (Bill Only) | No |
| LCHB | Id, Histoplasma/Blastomyces PCR | No, (Bill Only) | No |
| RMALF | Id MALDI-TOF Mass Spec Fungi | No, (Bill Only) | No |
| RMALY | Id MALDI-TOF Mass Spec Yeast | No, (Bill Only) | No |
| LCCA | Id, Candida auris Rapid PCR | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, reflex testing may be performed at an additional charge.
Method Name
Conventional Broth Culture/Macroscopic/Microscopic/D2 rDNA Gene Sequencing/Real-Time Polymerase Chain Reaction (PCR)/Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)
Dimorphic Pathogen Identification Confirmation: D2 rDNA Gene Sequencing/PCR/MALDI-TOF MS
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.