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HelpOrder Code LABFBL Fungal Culture, Blood
Additional Codes
Mayo Test Code: BFBL
EPIC Test Code: LABFBL
Reporting Name
Fungal Culture, BloodUseful For
Diagnosis and treatment of the etiologic agents of fungemia
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole bloodSpecimen Required
Container/Tube:
Preferred: Green top (sodium or lithium heparin)
Acceptable: SPS (sodium polyanethol sulfonate)
Specimen Volume: 5 mL
Pediatric Volume: 3 mL
Collection Instructions:
1. Send whole blood specimen in original tube. Do not aliquot.
2. SPS tubes are acceptable but not preferred.
Note: SPS tubes must be clearly labeled as SPS. If label is obscured, sample may be cancelled, as ACD (yellow top) is not an acceptable tube type.
Specimen Minimum Volume
Adults: 1 mL; Pediatrics: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | Ambient (preferred) | 7 days |
| Refrigerated | 7 days |
Reference Values
Negative
If positive, notification is made as soon as the positive culture is detected or identified.
Day(s) Performed
Monday through Sunday
CPT Code Information
87103-Blood
87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)
87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)
87107-Fungal identification panel A (if appropriate)
87107-Fungal identification panel B (if appropriate)
87153-D2 fungal sequencing identification (if appropriate)
87153- ITS Fungal Sequencing (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| FBL | Fungal Culture, Blood | 601-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| FBL | Fungal Culture, Blood | In Process |
Method Description
Blood is inoculated into a MycoF Lytic culture bottle (Becton Dickinson) bottle and continuously monitored on a blood culture instrument.
Identification of fungi is based on colonial and microscopic morphology, matrix-assisted laser desorption ionization time-of-flight mass spectrometry and/or D2 and internal transcribed spacer (ITS) rDNA gene sequencing, as applicable.(B; Dhiman N, Hall L, Wohlfiel SL, Buckwalter SP, Wengenack NL. Performance and cost analysis of matrix-assisted laser desorption ionization time of flight mass spectrometry for routine identification of yeast. J Clin Microbiol. 2011:49[4];1614-1616; Hall L, Wohlfiel SL, Roberts GD. Experience with the MicroSeq D2 large-subunit ribosomal DNA sequencing kit for identification of filamentous fungi encountered in the clinical laboratory. J Clin Microbiol. 2004;42[2]:622-626; Theel ES, Schmidt BH, Hall L, et al. Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2012;50[9]:3093-3095; Theel ES, Hall L, Mandrekar J, Wengenack NL. Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2011;49[12]:4067-4071; General approaches for direct and indirect detection and identification of fungi. In: Carroll KC, Pfaller MA, Pritt BS, et al. Manual of Clinical Microbiology. 13th ed. ASM Press; 2023)
Reject Due To
| Blood culture bottles (eg, BACTEC MycoF Lytic) | Reject |
| Isolator | Reject |
| Clotted | Reject |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| D2F | D2 Fungal Sequencing Identification | No, (Bill Only) | No |
| FUNA | Fungal Ident Panel A | No, (Bill Only) | No |
| FUNB | Fungal Ident Panel B | No, (Bill Only) | No |
| RMALF | Id MALDI-TOF Mass Spec Fungi | No, (Bill Only) | No |
| RMALY | Id MALDI-TOF Mass Spec Yeast | No, (Bill Only) | No |
| ITSF | ITS Fungal Sequencing | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, reflex testing may be performed at an additional charge.
Method Name
Conventional Broth Culture/Macroscopic/Microscopic/D2 and Internal Transcribed Spacer (ITS) rDNA Gene Sequencing/Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)
Dimorphic Pathogen Identification Confirmation: D2 and ITS rDNA Gene Sequencing/MALDI-TOF MS
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.