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HelpOrder Code LABGOR, B6PA Pyridoxic Acid, Plasma
Additional Codes
Mayo Test Code: B6PA
Method Name
Only orderable as part of a profile. For more information see B6PRO / Vitamin B6 Profile (Pyridoxal 5-Phosphate and Pyridoxic Acid), Plasma.
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Pyridoxic Acid (PA), PSpecimen Type
Plasma HeparinShipping Instructions
Ship specimen in amber vial to protect from light.
Specimen Required
Only orderable as part of a profile. For more information see B6PRO / Vitamin B6 Profile (Pyridoxal 5-Phosphate and Pyridoxic Acid), Plasma.
Patient Preparation:
1. Fasting: 12 hours, required; Infants-should have specimen collected before next feeding, water can be taken as needed
2. For 24 hours before specimen collection, patient must not take multivitamins or vitamin supplements.
Supplies: Amber Frosted Tube, 5 mL (T915)
Collection Container/Tube: Green top (sodium or lithium heparin) or plasma gel separator (PST)
Submission Container/Tube: Amber vial
Specimen Volume: 1 mL
Collection Instructions:
1. Within 2 hours of collection, centrifuge at 4° C.
2. Aliquot all plasma into amber vial and freeze immediately.
Specimen Minimum Volume
Plasma: 0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma Heparin | Frozen | 29 days | LIGHT PROTECTED |
Reference Values
Only orderable as part of a profile. For more information see B6PRO / Vitamin B6 Profile (Pyridoxal 5-Phosphate and Pyridoxic Acid), Plasma.
3-30 mcg/L
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82542
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| B6PA | Pyridoxic Acid (PA), P | 1688-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 61065 | Pyridoxic Acid (PA), P | 1688-1 |
Useful For
Determining vitamin B6 status, including in individuals who present with progressive nerve compression disorders, such as carpal tunnel and tarsal tunnel syndromes
Determining the overall success of a vitamin B6 supplementation program
Diagnosis and evaluation of hypophosphatasia
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Description
The stable isotope pyridoxal 5-phosphate-d3 and/or pyridoxic acid-d3 is added to plasma as an internal standard. Meta-phosphoric acid solution is then added to precipitate the proteins. Following sedimentation of the proteins, an aliquot of the clarified supernatant fluid is subjected to separation of pyridoxal 5-phosphate, pyridoxic acid, and internal standards from other plasma components by reverse-phase high-performance liquid chromatography with quantitation by tandem mass spectrometry.(Maus A, Girtman A, Kiesling J, Faber J, Grebe SKG. Overcoming the chromatographic challenges when performing LC-MS/MS measurements of pyridoxal-5'-phosphate. J Chromatogr B Analyt Technol Biomed Life Sci. 2023;1217:123605 doi:10.1016/j.jchromb.2023.123605)
Day(s) Performed
Monday through Thursday, Saturday, Sunday