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Order Code LABGOR, GPIC Glucose Phosphate Isomerase Enzyme Activity, Blood


Specimen Required


Only orderable as part of a profile. For more information see:

-HAEV1 / Hemolytic Anemia Evaluation, Blood

-EEEV1 / Red Blood Cell (RBC) Enzyme Evaluation, Blood


Useful For

Evaluating individuals with Coombs-negative chronic hemolysis as a part of a profile

Method Name

Only orderable as part of a profile. For more information see:

-HAEV1 / Hemolytic Anemia Evaluation, Blood

-EEEV1 / Red Blood Cell (RBC) Enzyme Evaluation, Blood

 

Kinetic Spectrophotometry (KS)

Reporting Name

Glucose Phosphate Isomerase, B

Specimen Type

Whole Blood ACD-B

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood ACD-B Refrigerated 20 days

Reject Due To

Gross hemolysis Reject
Fully clotted Reject

Reference Values

Only orderable as part of a profile. For more information see:

-HAEV1 / Hemolytic Anemia Evaluation, Blood

-EEEV1 / Red Blood Cell (RBC) Enzyme Evaluation, Blood

 

≥12 months of age: 40.0-58.0 U/g Hb

Reference values have not been established for patients younger than 12 months.

Method Description

Glucose phosphate isomerase (GPI) interconverts glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). In this assay, the F6P is then further converted to 6-phosphogluconate through the G6P dehydrogenase (G6PD) reaction resulting in the reduction of nicotinamide adenine dinucleotide phosphate (NADP[+]) to NADPH. The reduction of NADP(+) is measured spectrophotometrically by the increase in absorbance at 340 nm on an automated chemistry analyzer.(Beutler E. Red cell metabolism: A Manual of Biochemical Methods. 3rd ed. Grune and Stratton; 1984: 40-42; Rab MAE, van Wijk R. Enzymes of the red blood cell. In: Rifai N, Chiu RWK, Young I, Burnham CAD, Wittwer CT, eds. Tietz Textbook of Laboratory Medicine. 7th ed. Elsevier; 2023:chap 78)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84087

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GPIC Glucose Phosphate Isomerase, B 44050-3

 

Result ID Test Result Name Result LOINC Value
GPICL Glucose Phosphate Isomerase, B 44050-3

Day(s) Performed

Weekly