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HelpOrder Code LABGOR, HK1 Hexokinase Enzyme Activity, Blood
Specimen Required
Container/Tube:
Preferred: Yellow top (ACD solution B)
Acceptable: Lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Useful For
The evaluation of individuals with Coombs-negative chronic hemolysis
Method Name
Kinetic Spectrophotometry (KS)
Reporting Name
Hexokinase, BSpecimen Type
Whole Blood ACD-BSpecimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood ACD-B | Refrigerated | 20 days |
Reject Due To
| Gross hemolysis | Reject |
Reference Values
≥ 12 months: 0.7-1.7 U/g Hb
Reference values have not been established for patients who are younger than12 months.
Method Description
Hexokinase catalyzes the reaction of adenosine triphosphate (ATP) and glucose to glucose 6-phosphate (G6P) and adenosine diphosphate (ADP). In this assay the formation of G6P is measured by linking its further oxidation to 6-phosphogluconate to the reduction of nicotinamide adenine dinucleotide phosphate (NADP[+]) through the G6P dehydrogenase reaction. The increase in absorbance, which occurs as NADP(+) is reduced to NADPH, is measured spectrophotometrically at 340 nm on an automated chemistry analyzer.(Beutler E: Red Cell Metabolism: A Manual of Biochemical Methods. 3rd ed. Grune and Stratton; 1984:40-42; van Solinge WW, van Wijk: Enzymes of the red blood cell. In: Rifai N, Horvath AR, Wittwer CT: eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:chap 30)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82657
Day(s) Performed
Tuesday, Thursday
Forms
If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.