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Order Code LABGOR, KPLA Karius Spectrum, Plasma

Important Note

All Karius test requests must undergo a screening and receive approval or denial before the sample is collected. Please contact Laurie Benzer for more information.

Additional Codes

Mayo test Code: KLPA


Specimen Required


Collection Container/Tube:

Preferred: Plasma preparation tube (PPT)

Acceptable: Lavender top (K2 EDTA)

Submission Container/Tube:

Preferred: PPT

Acceptable: Sterile polypropylene tube

Specimen Volume: 1 mL Plasma

Collection Instructions :

1. Gently invert tube 8 to 10 times to mix whole blood.

2. Centrifuge specimen as follows:

 a. For PPT: Within 6 hours of collection, centrifuge at 1100 x g for 10 minutes.

 b. For K2 EDTA:

  i. Within 24 hours of collection, centrifuge at 1600 x g for 10 minutes. For tubes less than 4 mL, refer to tube manufacturer’s instructions for centrifugation speed and time.

  ii. Aliquot 1 mL of plasma into a sterile polypropylene tube, taking care not to disturb the buffy coat.

3. For complete instructions see Karius Spectrum Specimen Collection and Preparation Process.


Useful For

Rapid and minimally invasive detection of deep-seated and difficult-to-diagnose systemic infections throughout the body

 

Provide antimicrobial resistance detection for microbes known to utilize the antimicrobial resistance mechanism

Method Name

Metagenomic Sequencing

Reporting Name

Karius Spectrum, P

Specimen Type

Plasma

Specimen Minimum Volume

Plasma: 0.7 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Frozen (preferred) 180 days
  Ambient  4 days

Reject Due To

Incomplete or improper plasma separation Reject

Reference Values

An interpretive report will be provided.

Method Description

Karius Spectrum utilizes metagenomic sequencing of microbial-cell free DNA (mcfDNA) in plasma. The daily lab workflow involves preparing reagents and setting up automation systems for processing blood samples. Quality control materials are added to plasma samples to monitor issues such as contamination, technical errors, or sample mix-ups. DNA sequencing is performed directly from plasma without needing to extract DNA first, using a process tailored to recover microbial DNA efficiently. Each batch of samples is prepared together and sequenced using high-throughput instruments. Sequencing results are automatically analyzed and checked against background signals in real time. To improve the accuracy of clinical results, advanced bioinformatics filters are used, which help distinguish between true infections and background microbes commonly found in healthy individuals or due to environmental contamination. The filters are based on large reference datasets and known microbial patterns. Sequencing reads are matched to a comprehensive microbial reference database, and results are filtered to ensure they meet quality standards. The clinical reportable range includes over 1000 bacteria, DNA viruses, fungi and parasites known to be associated with human infections.

 

For a listing of pathogens see https://kariusdx.com/our-solution/pathogens?product=spectrum.

Day(s) Performed

Monday through Saturday

Performing Laboratory

Karius Laboratory

CPT Code Information

0152U

LOINC Code Information

Test ID Test Order Name Order LOINC Value
KPLA Karius Spectrum, P Not Provided

 

Result ID Test Result Name Result LOINC Value
KPLA Karius Spectrum, P Not Provided