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HelpOrder Code LABGOR, MB2GP Beta-2 Glycoprotein 1 Antibodies, IgM, Serum
Reporting Name
Beta 2 GP1 Ab IgM, SUseful For
Evaluating patients with suspected antiphospholipid syndrome by identification of beta-2 glycoprotein 1 IgM antibodies
First-line test when antiphospholipid syndrome is strongly suspected in conjunction with cardiolipin antibodies (IgG and IgM) and lupus anticoagulant testing
Estimating the risk of thrombosis and/or pregnancy-related morbidity in patients with systemic lupus erythematosus
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumAdditional Testing Requirements
Diagnostic criteria for antiphospholipid syndrome include the presence of at least one of the following: lupus anticoagulant, anticardiolipin, and anti-beta-2 glycoprotein 1 IgG or IgM antibodies. Consider ordering CLPMG / Phospholipid (Cardiolipin) Antibodies, IgG and IgM, Serum; GB2GP / Beta-2 Glycoprotein 1 Antibodies, IgG, Serum; and ALUPP / Lupus Anticoagulant Profile, Plasma concurrently with this test.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days |
Reference Values
<15.0 SMU (negative)
15.0-39.9 SMU (weakly positive)
40.0-79.9 SMU (positive)
≥80.0 SMU (strongly positive)
Results are reported in standard IgM anti-beta 2 glycoprotein 1 units (SMU).
Reference values apply to all ages.
Day(s) Performed
Monday through Saturday
CPT Code Information
86146
Method Description
Purified beta-2 glycoprotein 1 (B2GPI) antigen is bound to the wells of a polystyrene microwell plate under conditions that preserve the antigen in its native state. Prediluted controls and diluted patient sera are added to separate wells, allowing any B2GPI IgM antibodies present to bind to the immobilized antigen. Unbound sample is washed away, and an enzyme-labeled antihuman IgM conjugate is added to each well. A second incubation allows the enzyme-labeled antihuman IgM to bind to any patient antibodies that have attached to the microwells. After washing away any unbound enzyme-labeled antihuman IgM, the remaining enzyme activity is measured by adding a chromogenic substrate and measuring the intensity of the color that develops. The assay can be evaluated spectrophotometrically by measuring and comparing the color intensity that develops in the patient wells with that of a 5-point calibration curve. Semiquantitative results are reported out semiquantitatively in standard IgM anti-beta-2 GPI units (SMU).(Package insert: QUANTA Lite beta 2 GP1 IgM ELISA. Inova Diagnostics; Revision 16, 09/2018)
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat-treated specimens | Reject |
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)