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HelpOrder Code LABGOR, P5NT Pyrimidine 5' Nucleotidase, Blood
Reporting Name
Pyrimidine 5' Nucleotidase, BUseful For
Evaluation of marked basophilic stippling
Evaluation of hemolytic anemia
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole Blood ACD-BSpecimen Required
Specimen Type: Whole blood
Container/Tube:
Preferred: Yellow top (ACD solution B)
Acceptable: Lavender top (EDTA)
Specimen Volume: 5 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Specimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood ACD-B | Refrigerated | 20 days |
Reference Values
Normal
Day(s) Performed
Monday through Friday
CPT Code Information
83915
Method Description
Pyrimidine nucleotides have a spectral absorption curve that is markedly different from that exhibited by (normally present) adenine nucleotides (eg, adenosine triphosphate). The former have a peak at about 270 nm; the latter at about 257 nm. Thus, pyrimidine 5' nucleotidase deficiency may be ascertained by demonstrating a very high spectral absorption maximum of 270 nm in erythrocyte extracts.(Beutler E. Red Cell Metabolism. A Manual of Biochemical Methods. 3rd ed. Grune and Stratton; 1984:100-102; van Solinge WW, van Wijk. Enzymes of the red blood cell. In: Rifai N, Horvath AR, Wittwer CT: eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:chap 30)
Reject Due To
| Gross hemolysis | Reject |
Method Name
Kinetic Spectrophotometry
Forms
If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.