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Order Code LABGOR, PENTS Pentobarbital, Serum

Additional Codes

Mayo Test Code: PENTS

Reporting Name

Pentobarbital, S

Useful For

Monitoring of pentobarbital therapy treatment

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum Red


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Draw blood immediately before the next scheduled dose.

2. Within 2 hours of collection centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reference Values

Therapeutic range

Hypnotic: 1.0-5.0 mcg/mL

Therapeutic coma: 20-50 mcg/mL

Reducing intracranial pressure: 30-40 mcg/mL

This degree of sedation requires artificial respiratory support.

Toxic concentration: >10 mcg/mL

 

Cutoff concentrations by gas chromatography mass spectrometry:

Pentobarbital: 0.5 ng/mL

Day(s) Performed

Thursday

CPT Code Information

80299

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PENTS Pentobarbital, S 3924-8

 

Result ID Test Result Name Result LOINC Value
8239 Pentobarbital, S 3924-8

Method Description

Barbiturates are extracted from serum using solid-phase extraction techniques. The serum is buffered and eluted with organic solvent. The organic phase is dried, reconstituted, and the analysis performed by gas chromatography-mass spectrometry using selected ion monitoring. The assay utilizes deuterated barbiturates as internal standards.(Unpublished Mayo method)

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Method Name

Gas Chromatography Mass Spectrometry (GC-MS)

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.