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Order Code LABGOR, PKC Pyruvate Kinase Enzyme Activity, Blood


Specimen Required


Only available as part of a profile. For more information see:

HAEV1 / Hemolytic Anemia Evaluation, Blood

EEEV1 / Red Blood Cell (RBC) Enzyme Evaluation, Blood


Useful For

Evaluation of nonspherocytic hemolytic anemia as a part of a profile

 

Evaluation of neonatal anemia or jaundice

 

Evaluation of unexplained noninfectious hepatic failure

 

Evaluation of unexplained iron overload

 

Evaluation of unusually severe hemoglobin S trait

 

Evaluation of unusually severe glucose-6-phosphate dehydrogenase deficiency

 

Investigating families with pyruvate kinase deficiency to determine inheritance pattern and for genetic counseling

Method Name

Only available as part of a profile. For more information see:

HAEV1 / Hemolytic Anemia Evaluation, Blood

EEEV1 / Red Blood Cell (RBC) Enzyme Evaluation, Blood

 

Kinetic Spectrophotometry (KS)

Reporting Name

PK Enzyme Activity, B

Specimen Type

Whole Blood ACD-B

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood ACD-B Refrigerated 20 days

Reject Due To

Gross hemolysis Reject

Reference Values

Only available as part of a profile. For more information see:

HAEV1 / Hemolytic Anemia Evaluation, Blood

EEEV1 / Red Blood Cell (RBC) Enzyme Evaluation, Blood

 

≥12 months of age: 5.5-12.4 U/g Hb

Reference values have not been established for patients who are younger than 12 months.

Method Description

Pyruvate kinase catalyzes the phosphorylation of adenine diphosphate to adenine triphosphate by converting phosphoenolpyruvate to pyruvate. The amount of pyruvate formed is quantitated by adding lactate dehydrogenase and reduced nicotinamide adenine dinucleotide (NADH) and measuring the rate of decrease in absorbance spectrophotometrically at 340 nm as the NADH is oxidized to NAD(+) on an automated chemistry analyzer.(Beutler E: Red Cell Metabolism. In: A Manual of Biochemical Methods. 3rd ed. Grune and Stratton; 1984:68-71; van Solinge WW, van Wijk: Enzymes of the red blood cell. In: Rifai N, Horvath AR, Wittwer CT: eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:chap 30)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84220

Day(s) Performed

Monday through Friday