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Order Code LABGOR, VIBC Vibrio Culture, Feces

Additional Codes

Ordering Generic Mayo Test, LABGOR and then enter Mayo test code VIBC

Reporting Name

Vibrio Culture, Stool

Useful For

Determining whether Vibrio species may be the cause of diarrhea

 

This test is generally not useful for patients that have been hospitalized for more than 3 days because the yield from these patients' specimens is very low, as is the likelihood of identifying a pathogen that has not been detected previously.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Fecal


Additional Testing Requirements


1. If susceptibility testing is desired, also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies. If susceptibility testing is not needed (eg, due to lack of recovery of Vibrio species from feces), ZMMLS will not be performed and the order will be canceled.

2. In some cases, local public health requirements may impact Mayo Clinic Laboratories (MCL) clients, requiring submission of isolates to public health laboratories. Clients should familiarize themselves with local requirements and are responsible for submitting isolates to appropriate public health laboratories. Clients can obtain isolates of Vibrio species recovered from specimens submitted to MCL by calling 800-533-1710 as soon as possible after reporting to ensure viability of the bacterium.



Shipping Instructions


Specimen must arrive within 96 hours of collection.



Necessary Information


Specimen source is required.



Specimen Required


Patient Preparation: Medications: Do not use barium or bismuth before collection of specimen.

Supplies: Culture and Sensitivity Stool Transport Vial (T058)

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: Representative portion of fecal specimen

Collection Instructions:

1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

3. Place vial in a sealed plastic bag.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Fecal Ambient (preferred) 4 days
  Refrigerated  4 days

Reference Values

No growth of pathogen

Day(s) Performed

Monday through Sunday

CPT Code Information

87046-Vibrio culture, stool

87077-Identification by MALDI-TOF mass spec (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

Method Description

The fecal specimen is inoculated onto selective media, thiosulfate-citrate-bile salts-sucrose agar (TCBS), designed to inhibit growth of normal bowel flora while allowing growth of Vibrio species. After incubation, suspect colonies are identified using 1 or a combination of the following techniques: matrix-associated laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, conventional biochemical tests, carbon source utilization, and nucleic acid sequencing of the 16S ribosomal RNA (rRNA) gene.(Tarr CL, Glover WAII, Boucher Y, et al: Chapter 43: Vibrio and Related Organisms. In Manual of Clinical Microbiology. 12th edition. Edited by KC Carroll, MA Pfaller. Washington DC,ASM Press, 2019, pp 775-786)

Reject Due To

Fecal swab
Feces in Ecofix (green cap)
Specimen in buffered glycerol saline transport media
Reject

Method Name

Conventional Culture

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged separately.