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Order Code LABHGSC Sickle Solubility, Blood

Additional Codes

Mayo Test ID:  SDEX

Reporting Name

Sickle Solubility, B

Useful For

Screening for presence or absence of hemoglobin (Hb) S (sickle cell disease)

Specimen Type

Whole Blood EDTA


Ordering Guidance


This is a screening test only. For quantification of hemoglobin S, order HBEL1 / Hemoglobin Electrophoresis Evaluation, Blood.



Necessary Information


1. Patient's age is required.

2. Include recent transfusion information.



Specimen Required


Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD solution B), green top (heparin)

Specimen Volume: 1 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in the original tube. Do not aliquot.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Refrigerated 14 days

Reject Due To

Gross hemolysis Reject

Reference Values

Negative

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Day(s) Performed

Monday through Friday

Method Name

Hemoglobin S Solubility

CPT Code Information

85660

Method Description

Hemoglobin S reduced by dithionite is insoluble in concentrated inorganic buffers, and thus, the solution is so turbid that newsprint cannot be read through a tube containing the hemolysate and reagents.(Fairbanks VF, Klee GG: Biochemical aspects of hematology. In: Burtis CA, Ashwood ER, eds. Tietz Textbook of Clinical Chemistry. 3rd ed. WB Saunders Company; 1999: 1678-1679; Greene DN, Vaughn CP, Crews BO, Agarwal AM. Advances in the detection of hemoglobinopathies. Clinica Chimica Acta. 2015; 439:50-57)

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.