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Order Code LABLNORO Norovirus PCR, Molecular Detection, Feces

Additional Codes

Mayo Test Code: LNORO
EPIC Test Code: LABLNORO

Useful For

Diagnosing gastrointestinal disease (diarrhea or vomiting) caused by norovirus genogroups 1 and 2

 

This test should not be used as a test-of-cure.

Method Name

Real-Time Polymerase Chain Reaction (PCR)/RNA Probe Hybridization

Reporting Name

Norovirus PCR, F

Specimen Type

Fecal


Shipping Instructions


Place vial in a sealed plastic bag and ship ambient. Specimens received at other temperatures will be rejected, and testing will be canceled.



Specimen Required


Supplies: Culture and Sensitivity Stool Transport Vial (T058)

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium - either Cary-Blair, Para-Pak Culture and Sensitivity Media)

Specimen Volume: Representative portion of diarrheal fecal sample, 1 gram or 5 mL

Collection Instructions:

1. Collect fresh feces and place in preservative within 1 hour of collection.

2. Visibly formed feces are not consistent with Norovirus gastrointestinal disease and should not be submitted for testing.


Specimen Stability Information

Specimen Type Temperature Time
Fecal Ambient 7 days

Reject Due To

Nonpreserved feces
Transport media other than Cary-Blair or C and S
Modified Cary-Blair
Visibly formed feces		 Reject

Reference Values

Negative

Method Description

This assay utilizes real-time, TaqMan-based polymerase chain reaction (PCR) technology to target the nonstructural polyprotein gene of norovirus G1 and G2. Nucleic acid is extracted from diarrheal stool samples in Cary-Blair media. Following extraction of viral RNA, reverse transcription is performed to convert norovirus genomic RNA to complementary DNA. Real-time amplification and detection are then performed. Two separate real-time PCR reactions are performed for each sample, one specific for norovirus G1 and the second targeting norovirus G2. The assay is able to accurately detect and differentiate these genogroups in clinical stool samples.(Unpublished Mayo method)

Day(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798 x 2