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HelpOrder Code LABLPBF1 Lipase, Body Fluid
Necessary Information
1. Date and time of collection are required.
2. Specimen source is required.
Specimen Required
Specimen Type: Body fluid
Preferred Sources:
-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)
-Pleural fluid (pleural, chest, thoracentesis)
-Drain fluid (drainage, Jackson Pratt [JP] drain)
-Pericardial
Acceptable Source: Write in source name with source location (if appropriate)
Collection Container/Tube: Sterile container
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge to remove any cellular material and transfer into a plastic vial.
2. Indicate the specimen source and source location on label.
Useful For
Determining whether pancreatic inflammation or pancreatic fistula may be contributing to a pathological accumulation of fluid
Method Name
Enzymatic Colorimetric Reaction
Reporting Name
Lipase, BFSpecimen Type
Body FluidSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Body Fluid | Refrigerated (preferred) | 7 days |
| Frozen | 30 days | |
| Ambient | 24 hours |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Cerebrospinal fluid, feces, breast milk, saliva, nasal secretions, gastric secretions, bronchoalveolar lavage (BAL) or bronchial washings, sputum, synovial, colostomy/ostomy, urine, or vitreous fluid | Reject |
Reference Values
An interpretive report will be provided.
Method Description
The chromogenic lipase substrate 1,2-O-dilauryl-rac-glycero-3-glutaric acid-(6-methylresorufin) ester is cleaved by the catalytic action of alkaline lipase solution to form 1,2-O-dilauryl-rac-glycerol and an unstable intermediate, glutaric acid-(6-methylresorufin) ester.
This decomposes spontaneously in alkaline solution to form glutaric acid and methylresorufin. Addition of detergent and colipase increases the specificity of the assay for pancreatic lipase.(Package insert: Roche Lipase reagent. Roche Diagnostics; V 3.0, 03/2022)
Day(s) Performed
Monday through Sunday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
83690