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Order Code LABMANDIFFCV - Lab Use Only Differential Count, Manual, Blood

Additional Codes

Test Name in EPIC

EPIC Test Code

Mnemonic

RV Manual Differential (CellaVision)L

LABMANDIFFCV

Manual Diff

 

Useful For

The blood differential test can detect abnormal or immature cells.

Specimen Requirements

Specimen Type: Whole Blood

Collection Container/Tube: Lavender (EDTA)

Pediatric Collection Container/Tube: Lavender Microtainer (EDTA)

Specimen Volume: optimal 2 mL, minimum 1 mL

Pediatric (microtainer) volume: 0.5 mL

 

Whole blood specimen should be inverted gently several times to mix blood immediately after collection. 

  • If sending to RMC/RSMC: Send EDTA tube refrigerated and print out results. (Clinic specimens needing follow-up.)
  • If sending to RMC from TTMC/RSMC: Send EDTA tube and print out with manual diff/reasons for pathology. Include 1 stained slide and 1 unstained slide.

 

Peripheral Blood Smear Instructions:

  1. Use clean, high-quality glass slides
  2. Using EDTA blood, drop a blood droplet that is about 4mm in diameter
  3. Adjust spreading speed and angle (30-40 degrees) until 2 slides are adequate with smear approximately ¾ length of glass slide
  4. The smear should have a smooth feathered edge.
  5. Label thick end of smears with patient’s full name, date of birth, and date of collection.
  6. Important - slides must be completely dry prior to putting them into a slide container. Send slides refrigerated or ambient.
  7. If sending EDTA whole blood for RMC/RSMC to prepare blood smears, specimen must arrive within 12 hours of draw.

Specimen Stability

Specimen Type Temperature Stability         
Whole Blood Refrigerated 48 hours
Slides/Smears Ambient Indefinitely

Reference Values

Gender abbreviations (M=Male, F=Female, U=Unknown, Unspecified, X=Neither Male nor Female)

 

Platelets:

All ages, all genders         150 - 450 x 103/uL

 

WBC:
​​​

Age/
gender		 WBC
#x103/uL		 PMN
%		 PMN
#x103/uL 		 Lymph
%		 Lymph
#x103/uL 		 Mono
%		 Mono
#x103/uL 		 Eos
%		 Eos
#x103/uL 		 Baso
%		 Baso
#x103/uL		 IG
%		 IG
#x103/uL 		 **Mixed
%		 **Mixed
#x103/uL 		 NRBCs
#/100
0-3 days
M,F,U,X		 9.0-35.0 32-62 2.88-28.0 19-29 1.71-10.15 6.0-18 0.54-6.3 0-2 0.0.7 0-1 0-0.35 * * 5.0-10   0-2
4-9 days
M,F,U,X		 5.0-21.0 19-49 0.95-13.44 26-36 1.3-7.56 7.0-18 0.35-3.78 0-2 0-0.42 0-1 0-0.21 * * 5.0-10   0-2
10-14 days
M,F,U,X		 5.0-20.0 14-34 0.7-9.6 36-45 1.8-9.0 4.0-18 0.2-3.6 0-2 0-0.4 0-1 0-0.2 * * 6.0-13   0
15-30 days
M,F,U,X		 5.0-19.5 15-35 0.75-9.17 43-53 2.15-10.34 4.0-16 0.2-3.12 0-2 0-0.195 0-1 0-0.2 * * 7.0-14   0
1-6 mo
M,F,U,X		 6.0-17.5 13-33 0.78-7.7 41-71 2.46-12.43 4.0-14 0.24-2.45 0-3 0-0.53 0-1 0-0.18 * * 4.0-11   0
7-24 mo
M,F,U,X		 6.0-17.0 15-35 0.9-7.82 45-76 2.7-12.92 4.0-12 0.24-2.04 0-3 0-0.51 0-1 0-0.17 * * 3.0-10   0
25-60 mo
M,F,U,X		 5.5-15.5 23-45 1.27-8.68 35-65 1.93-10.08 4.0-10 0.22-1.55 0-3 0-0.47 0-1 0-0.16 * * 3.0-10   0
5-8 years
M,F,U,X		 5.0-14.5 32-54 1.6-9.43 28-48 1.4-6.96 4.0-10 0.2-1.45 0-3 0-0.44 0-1 0-0.15 * * 3.0-10   0
9-12 years
M,F,U,X		 4.5-13.5 33-61 1.49-9.72 28-48 1.26-6.48 4.0-10 0.18-1.35 0-3 0-0.41 0-1 0-0.14 * * 3.0-10   0
13-18 years
M,F,U,X		 4.5-13.0 34-64 1.53-9.75 25-45 1.13-5.85 4.0-10 0.18-1.30 0-3 0-0.39 0-1 0-0.13 * * 3.0-10   0
≥ 18 years
M,F,U,X		 3.5-11.0 39-75 1.7-7.5 17-47 1.0-4.8 3.0-13 0.2-0.9 0-5 0-0.5 0-1 0-0.3 0-1 0-0.1 3.0-19 0.04-1.64 0

*No established ranges for pediatrics
**Only run on XN

Day(s) Test Set Up

Monday through Sunday

Performing Laboratory

Ridgeview Medical Center Laboratory

 

Methodology

Microscopy; includes Neutrophils, lymphocytes, monocytes, eosinophils, basophils, bands, blasts, metamyelocytes, myelocytes, promyelocytes, RBC morphology, and platelet morphology.

Test Classification and CPT Coding

85007

Rejection Criteria

  1. Samples that do not meet minimum volume requirements
  2. Incorrect collection container
  3. Clotted or visibly hemolyzed