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Order Code LABMTRNA Trichomonas vaginalis, Nucleic Acid Amplification, Varies

Additional Codes

Mayo Test Code: MTRNA
EPIC Test Code: LABMTRNA

Useful For

Detecting Trichomonas vaginalis in prostatic massage (VBIII) fluid or male urethral swabs

Reporting Name

T.vaginalis, Misc, Amplified RNA

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


This test should only be performed in men.

 

Submit only 1 of the following specimens:

 

Specimen Type: Post-prostatic massage fluid (VBIII)

Supplies: Aptima Urine Transport Tube (T582)

Container/Tube: Aptima Urine Specimen Transport Tube

Specimen Volume: 15 to 20 mL

Collection Instructions:

1. Patient should not have urinated for at least 1 hour prior to specimen collection.

2. Patient should void a small amount of urine prior to prostatic massage. Pre-massage urine can be discarded or submitted for other testing as applicable.

3. Patient then ceases voiding and a prostatic massage is performed by the urologist or other health care professional.

4. Collect post-massage fluid into a sterile, plastic, preservative-free container.

5. Transfer 2 mL of post-massage fluid specimen into the Aptima urine specimen transport tube using the disposable pipette provided within 24 hours of collection. The correct volume of fluid has been added when the fluid level is between the black fill lines on the Aptima urine transport tube.

 

Specimen Type: Urethral (male only)

Supplies: Aptima Unisex Swab Collection Kit (T583)

Container/Tube: Aptima Unisex Swab

Specimen Volume: Swab

Collection Instructions:

1. Urethral specimens must be collected using an Aptima Unisex Swab Collection kit.

2. Patient should not have urinated for at least 1 hour prior to collection.

3. With a rotating movement, insert swab (blue shaft) 2 to 4 cm into urethra.

4. Once inserted, rotate blue swab gently at least 1 full rotation using sufficient pressure to ensure swab comes into contact with all urethral surfaces. Allow blue swab to remain inserted for 2 to 3 seconds.

5. Place blue swab in the Aptima transport tube provided in collection kit.

6. Snap off blue swab at score line so it fits into closed tube.

7. Cap tube securely and label tube with patient's entire name and collection date and time.


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 30 days APTIMA VIAL
  Ambient  30 days APTIMA VIAL

Reject Due To

Transport tubes containing a cleaning swab or more than 1 swab
Multiple sources on single tube
Reject

Reference Values

Negative

Method Description

The APTIMA Trichomonas vaginalis Assay combines the technologies of target capture, transcription-mediated amplification, and hybridization protection assay for detection of 16S ribosomal RNA from T vaginalis.(Package insert: Aptima Trichomonas vaginalis Assay. AW-27552-001, Hologic, Inc; Rev. 002, 12/2023)

Day(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87661

Method Name

Transcription-Mediated Amplification

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.