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Order Code LABPAP PAP Smear with Reflex to HPV if ASCUS

Specimen Requirements

Include date of birth, medical record number (or other identification number), previous malignancy, drug therapy, radiation therapy, last menstrual period (LMP), postmenopausal patient (PMP), surgery (including surgical biopsies), exogenous hormones, abnormal vaginal bleeding, abnormal Pap results, IUD, and all other pertinent clinical information on the cytology request form.

 

Sample Collection Using BD SurePath Vial and Cervical Sampling Device with Detachable Head:

1. Obtain a sample from the cervix according to the standard collection procedure provided by BD SurePath.

2. Using the thumb and forefinger of gloved hand, disconnect the head of the device from the handle, insert the head in the collection vial.  Discard the handle of the sampling device.  Do not touch the head of the device.

3. Cap the vial tightly

4. Send the specimen containing the head of the sampling device, with appropriate paperwork, to the laboratory for processing.

 

Failure to obtain adequate ectocervical, endocervical, or vaginal cell population is suboptimal for evaluation. Excessive use of lubricating jelly on the vaginal speculum will interfere with cytologic examination and may lead to unsatisfactory Pap results.

Reference Values

PAP:

Result will be reported using The Bethesda System for Reporting Cervical/Vaginal Cytologic Diagnosis. 

HPV:

Result will be reported using Roche Cobas 4800.  This test detects only the 2 most common high-risk HPV types (16 and 18) and any other type will be included into ‘other’ category and the specific HPV type present cannot be determined. 

Day(s) Test Set Up

Monday through Friday

Please call TTMC Lab for HPV specimen TAT information

Performing Laboratory

PAP:

Ridgeview Medical Center Laboratory- Cytology Department

HPV:

Two Twelve Medical Center Laboratory

Methodology

Liquid-based PAP with molecular amplification and detection of HPV

 

Specimen Stability Information

BD SurePath Vial Ambient 21 days
  Refrigerated No
  Frozen No