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Order Code LABPLHBB Plasma Free Hemoglobin, Plasma

Reporting Name

Plasma Free Hemoglobin, P

Useful For

Determining whether hemolysis is occurring such as from:

-Transfusion reaction

-Mechanical fragmentation of red blood cells

-Relative comparison to baseline levels in extracorporeal membrane oxygenation and centrifugal ventricular assist device patients to assess pump disruption

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Plasma EDTA


Specimen Required


Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions:

1. Centrifuge and transfer plasma to a plastic vial within 2 hours of collection.

2. Results could be falsely elevated due to artifactual red blood cell lysis if not centrifuged within 2 hours of collection.


Specimen Minimum Volume

1.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Refrigerated (preferred) 20 days
  Frozen  30 days
  Ambient  4 days

Reference Values

TOTAL PLASMA HEMOGLOBIN

≥12 months: 0.0-15.2 mg/dL

Reference values have not been established for patients who are younger than 12 months of age.

 

OXYHEMOGLOBIN

≥12 months: 0.0-12.4 mg/dL

Reference values have not been established for patients who are younger than 12 months of age.

Day(s) Performed

Monday through Sunday

CPT Code Information

83051

Method Description

Plasma is examined spectrophotometrically at 8 wavelengths ranging from 415 to 700 nm, and calculations are made that permit quantitation of total heme pigments, oxyhemoglobin, total bilirubin, and methemalbumin, comprising all the frequently encountered plasma pigments. Linearity studies have enabled reporting of values to 2000 mg/dL.(Fairbanks VF, Ziesmer S, O'Brien PC. Methods for measuring plasma hemoglobin in micromolar concentration compared. Clin Chem. 1992;38(1):132-140; Neal JR, Quintana E, Pike RB, Hoyer JD, Joyce LD, Schears G. Using daily plasma-free hemoglobin levels for diagnosis of critical pump thrombus in patients undergoing ECMO or VAD support. J Extra Corpor Technol. 2015;47[2]:103-108)

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Method Name

Spectrophotometry (SP)

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.