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Order Code LABPLP Pyridoxal 5-Phosphate, Plasma

Important Note

For Vitamin B6 testing it is  appropriate to follow one of the processes below in lieu of using a refrigerated centrifuge-

 

  1. Place centrifuge inserts in the freezer to keep them cold.  Use these inserts to centrifuge specimen within 2 hours of collection. Immediately aliquot all plasma into amber vial and refrigerate.

 

  1. Refrigerate specimen after collection until well chilled. Within 2 hours of collection centrifuge specimen and  immediately aliquot all plasma into amber vial and refrigerate.

 

Additional Codes

MAYO CODE: PLP
EPIC Order Code: LABPLP

Useful For

Determining the concentration of pyridoxal 5-phosphate in the assessment of vitamin B6 status

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Pyridoxal 5-Phosphate (PLP), P

Specimen Type

Plasma Heparin


Shipping Instructions


Ship specimen in amber vial to protect from light.



Specimen Required


Patient Preparation:

1. Patient should fast overnight (12-14 hours); infants-should have specimen collected before next feeding. Water can be taken as needed.

2. For 24 hours before specimen collection, patient must not take multivitamins or vitamin supplements.

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube: Green top (sodium or lithium heparin) or plasma gel separator (PST)

Submission Container/Tube: Amber vial

Specimen Volume: 1 mL

Collection Instructions:

1. Centrifuge at 4° C within 2 hours of collection.

2. Aliquot all plasma into amber vial and freeze immediately.


Specimen Minimum Volume

0.75 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Heparin Frozen 29 days LIGHT PROTECTED

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

5-50 mcg/L

Day(s) Performed

Monday through Thursday, Saturday, Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84207

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.

Method Description

The stable isotope pyridoxal 5-phosphate-d2 and/or pyridoxic acid-d2 is added to plasma as an internal standard. Meta-phosphoric acid solution is then added to precipitate the proteins. Following sedimentation of the proteins, an aliquot of the clarified supernatant fluid is subjected to separation of pyridoxal 5-phosphate, pyridoxic acid, and internal standards from other plasma components by reverse-phase high-performance liquid chromatography with quantitation by tandem mass spectrometry.(Unpublished Mayo method)