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Order Code LABPOWV Powassan Virus, IgM, Enzyme-Linked Immunosorbent Assay, Serum

Additional Codes

Mayo Test Code: POWV

EPIC Test Code: LABPOWV

 


Ordering Guidance


For patients with less than 7 days of symptoms, the recommended testing is molecular analysis for detection of Powassan virus (POWV) RNA. Contact either a local Public Health Laboratory or the Centers for Disease Control and Prevention.



Specimen Required


Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Diagnosis of Powassan virus infection.

 

This test should not be used as a screening procedure for the general population.

 

This test should not be used as a "test of cure."

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Powassan Virus, IgM, ELISA, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  30 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat inactivated specimen Reject

Reference Values

Negative

Reference values apply to all ages.

Method Description

The test uses microtiter strips, each with 8 break-off reagent wells coated with recombinant Powassan virus antigens. In the first reaction step, diluted patient samples are incubated in the wells. In the case of positive samples, specific IgM (also IgA and IgG) antibodies will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labelled anti-human IgM (enzyme conjugate) catalyzing a color reaction.(Unpublished Mayo method)

Day(s) Performed

Tuesday, Thursday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86790

Testing Algorithm

For information see the following:

-Meningitis/Encephalitis Panel Algorithm

-Acute Tickborne Disease Testing Algorithm

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.