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HelpOrder Code LABPSAU Prostate-Specific Antigen (PSA) Ultrasensitive, Serum
Additional Codes
Mayo Test Code: PSAU
EPIC Test Code: LABPSAU
Useful For
As an aid in the detection of prostate cancer when used in conjunction with a digital rectal exam in men ages 50 years and older
To aid in the prognosis and management of individuals diagnosed with prostate cancer
Monitoring disease after radical prostatectomy
This test should not be used for initial prostate cancer screening.
Method Name
Electrochemiluminescent Immunoassay (ECLIA)
Reporting Name
PSA, Ultrasensitive, SSpecimen Type
SerumOrdering Guidance
Free prostate-specific antigen (PSA) can be added on within 72 hours of performing this test. Specimen must have been shipped frozen.
If both free and total PSA results are desired, order PSAFT / Prostate-Specific Antigen (PSA), Total and Free, Serum.
Necessary Information
Include patient's age.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Serum gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial within 2 hours of collection.
Specimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 180 days |
| Refrigerated | 14 days | |
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
Males:
|
Age (years) |
PSA upper limit (ng/mL) |
|
<40 |
≤2.0 |
|
40-49 |
≤2.5 |
|
50-59 |
≤3.5 |
|
60-69 |
≤4.5 |
|
70-79 |
≤6.5 |
|
≥80 |
≤7.2 |
Females: Not applicable
Method Description
The Roche Elecsys total PSA (prostate-specific antigen) assay is a sandwich electrochemiluminescence immunoassay that employs a biotinylated monoclonal PSA-specific antibody and a monoclonal PSA-specific antibody labeled with ruthenium complex. PSA in the specimen reacts with both the biotinylated monoclonal PSA-specific antibody (mouse) and the monoclonal PSA-specific antibody (mouse) labeled with a ruthenium, forming a sandwich complex. Streptavidin-coated microparticles are added and the mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell. Application of voltage to the electrode induces the chemiluminescent emission, which is then measured against a calibration curve to determine the amount of PSA in the patient specimen. This method has been standardized against the Reference Standard/WHO 96/670.(Package insert: Elecsys total PSA. Roche Diagnostics; V 2.0, 06/2023)
Day(s) Performed
Monday through Saturday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84153