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Order Code LABSLIDE - Lab Use Only Slide Review

Additional Codes

Test Name in EPIC

EPIC Test Code           

Mnemonic

RV SLIDE REVIEW

LABSLIDE

SMEAR

 

Useful For

The slide review test can detect abnormal RBC and PLT Morphologies.

Specimen Requirements

Specimen Type: Whole Blood

Collection Container/Tube: Lavender (EDTA)

Pediatric Collection Container/Tube: Lavender Microtainer (EDTA)

Specimen Volume: optimal 2 mL, minimum 1 mL

Pediatric (microtainer) volume: 0.5 mL

 

Whole blood specimen should be inverted gently several times to mix blood immediately after collection. 

  • If sending to RMC/RSMC: Send EDTA tube refrigerated and print out results. (Clinic specimens needing follow-up.)
  • If sending to RMC from TTMC/RSMC: Send EDTA tube and print out with manual diff/reasons for pathology. Include 1 stained slide and 1 unstained slide.

 

Peripheral Blood Smear Instructions:

  1. Use clean, high-quality glass slides
  2. Using EDTA blood, drop a blood droplet that is about 4mm in diameter
  3. Adjust spreading speed and angle (30-40 degrees) until 2 slides are adequate with smear approximately ¾ length of glass slide
  4. The smear should have a smooth feathered edge.
  5. Label thick end of smears with patient’s full name, date of birth, and date of collection.
  6. Important - slides must be completely dry prior to putting them into a slide container. Send slides refrigerated or ambient.
  7. If sending EDTA whole blood for RMC/RSMC to prepare blood smears, specimen must arrive within 12 hours of draw.

Specimen Stability

Specimen Type Temperature Stability         
Whole Blood Refrigerated (Perferred) 48 hours
Whole Blood Ambient 24 hours
Slides/Smears Ambient Indefinitely

Reference Values

Gender abbreviations (M=Male, F=Female, U=Unknown, Unspecified, X=Neither Male nor Female)

 

Platelets:

All ages, all genders         150 - 450 x 103/uL

 

WBC:
 

Age/
gender		 WBC
#x103/uL		 PMN
%		 PMN
#x103/uL 		 Lymph
%		 Lymph
#x103/uL 		 Mono
%		 Mono
#x103/uL 		 Eos
%		 Eos
#x103/uL 		 Baso
%		 Baso
#x103/uL		 IG
%		 IG
#x103/uL 		 **Mixed
%		 **Mixed
#x103/uL 		 NRBCs
#/100
0-3 days
M,F,U,X		 9.0-35.0 32-62 2.88-28.0 19-29 1.71-10.15 6.0-18 0.54-6.3 0-2 0.0.7 0-1 0-0.35 * * 5.0-10   0-2
4-9 days
M,F,U,X		 5.0-21.0 19-49 0.95-13.44 26-36 1.3-7.56 7.0-18 0.35-3.78 0-2 0-0.42 0-1 0-0.21 * * 5.0-10   0-2
10-14 days
M,F,U,X		 5.0-20.0 14-34 0.7-9.6 36-45 1.8-9.0 4.0-18 0.2-3.6 0-2 0-0.4 0-1 0-0.2 * * 6.0-13   0
15-30 days
M,F,U,X		 5.0-19.5 15-35 0.75-9.17 43-53 2.15-10.34 4.0-16 0.2-3.12 0-2 0-0.195 0-1 0-0.2 * * 7.0-14   0
1-6 mo
M,F,U,X		 6.0-17.5 13-33 0.78-7.7 41-71 2.46-12.43 4.0-14 0.24-2.45 0-3 0-0.53 0-1 0-0.18 * * 4.0-11   0
7-24 mo
M,F,U,X		 6.0-17.0 15-35 0.9-7.82 45-76 2.7-12.92 4.0-12 0.24-2.04 0-3 0-0.51 0-1 0-0.17 * * 3.0-10   0
25-60 mo
M,F,U,X		 5.5-15.5 23-45 1.27-8.68 35-65 1.93-10.08 4.0-10 0.22-1.55 0-3 0-0.47 0-1 0-0.16 * * 3.0-10   0
5-8 years
M,F,U,X		 5.0-14.5 32-54 1.6-9.43 28-48 1.4-6.96 4.0-10 0.2-1.45 0-3 0-0.44 0-1 0-0.15 * * 3.0-10   0
9-12 years
M,F,U,X		 4.5-13.5 33-61 1.49-9.72 28-48 1.26-6.48 4.0-10 0.18-1.35 0-3 0-0.41 0-1 0-0.14 * * 3.0-10   0
13-18 years
M,F,U,X		 4.5-13.0 34-64 1.53-9.75 25-45 1.13-5.85 4.0-10 0.18-1.30 0-3 0-0.39 0-1 0-0.13 * * 3.0-10   0
≥ 18 years
M,F,U,X		 3.5-11.0 39-75 1.7-7.5 17-47 1.0-4.8 3.0-13 0.2-0.9 0-5 0-0.5 0-1 0-0.3 0-1 0-0.1 3.0-19 0.04-1.64 0

*No established ranges for pediatrics
**Only run on XN

 

RBC:
 

Age/
Gender		 RBC # x 106/uL Hgb g/dL HCT % MCV fL MCH pg MCHC g/dL RDW-CV %
0-6 days
M,F,U,X		 4.00-6.60 14.5-22.5 45-67 95-121 31-37 29-37 13.0-180
7-13 days
M,F,U,X		 3.90-6.30 13.5-19.5 42-66 88-126 28-40 28-38 13.0-18.0
14 days - 1 mo
M,F,U,X		 3.60-6.20 12.5-20.5 39-63 86-124 28-40 28-38 13.0-18.0
1-2 mo
M,F,U,X		 3.00-5.40 10.0-18.0 31-55 85-123 28-40 29-37 11.5-16.0
2-3 mo
M,F,U,X		 2.70-4.90 9.0-14.0 28-42 77-115 26-34 29-37 11.5-16.0
3-6 mo
M,F,U,X		 3.10-4.50 9.5-13.5 29-41 74-108 25-35 30-36 11.5-16.0
6 mo - 2 years
M,F,U,X		 3.70-5.30 10.5-13.5 33-49 70-86 23-31 30-36 11.5-16.0
2-6 years
M,F,U,X		 3.90-5.30 11.5-15.5 34-40 75-87 24-30 32-36 11.5-15.0
6-12 years
M,F,U,X		 4.00-5.20 13.0-16.0 35-45 77-95 25-33 32-36 11.5-15.0
12-18 years
M		 4.50-5.30 12.0-15.5 36-51 79-98 25-35 32-36 11.5-14.0
12-18 years
F		 4.10-5.10 12.0-16.0 33-51 78-102 25-35 32-36 11.5-14.0
12-18 years
U,X		 4.10-5.30 12.0-16.0 33-51 78-102 25-35 32-36 11.5-14.0
≥ 18 years
M		 4.32-6.00 13.5-18.0 38.8-52 80-100 27-34 31.5-35.2 11.5-15.5
≥ 18 years
F		 3.80-5.20 12.0-15.5 35.0-47 80-100 27-34 31.5-35.2 11.5-15.5
≥ 18 years
U,X		 3.80-6.00 12.0-18.0 35.0-52 80-100 27-34 31.5-35.2 11.5-15.5

Day(s) Test Set Up

Monday through Sunday

Performing Laboratory

Ridgeview Medical Center Laboratory

Two Twelve Medical Center Laboratory

Ridgeview Sibley Medical Center Laboratory

Methodology

Microscopy; includes RBC morphology, and platelet morphology.

Test Classification and CPT Coding

No Charge

Rejection Criteria

  1. Samples that do not meet minimum volume requirements
  2. Incorrect collection container
  3. Clotted or visibly hemolyzed