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Order Code LAD1 Leukocyte Adhesion Deficiency Type 1, CD11a/CD18 and CD11b/CD18 Complex Immunophenotyping, Blood

Reporting Name

Leukocyte Adhesion Def. Type 1, B

Useful For

Aiding in the diagnosis of leukocyte adhesion deficiency syndrome type 1, primarily in patients younger than 18 years

 

CD11a, CD11b, and CD18 phenotyping

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole Blood EDTA


Shipping Instructions


Testing is performed Monday through Friday. Specimens not received by 4 p.m. (CST) on Friday may be canceled.

 

Draw and package specimen as close to shipping time as possible. It is recommended that specimens arrive within 24 hours of draw.

 

Specimens arriving on the weekend and observed holidays may be canceled.



Necessary Information


Date and time of collection and healthcare professional name and phone number are required.



Specimen Required


Container/Tube: Lavender top (EDTA)

Specimen Volume: 5 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.

Additional Information: For serial monitoring, it is recommended that specimens are collected at the same time of day.


Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient 72 hours PURPLE OR PINK TOP/EDTA

Reference Values

Normal (reported as normal or absent expression for each marker)

Day(s) Performed

Monday through Friday

CPT Code Information

86356 x 3

Method Description

Flow cytometric immunophenotyping of peripheral blood is performed to evaluate the presence or absence of the CD11/CD18 complex using monoclonal antibodies directed against the CD11 isoforms, CD11a and CD11b, and CD18 antigens.(O'Gorman MR, McNally AC, Anderson DC, et al. A rapid whole blood lysis technique for the diagnosis of moderate or severe leukocyte adhesion deficiency [LAD]. Ann NY Acad Sci. 1993;677:427-430)

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Method Name

Flow Cytometric Immunophenotyping

Genetics Test Information

ITGB2 is located on the long arm of chromosome 21(21q22.3). It encodes the common beta 2 integrin subunit (CD18), which is necessary for the expression of lymphocyte function-associated antigen 1 (CD11a/CD18), Mac-1/CR3 (CD11b/CD18), and p150/95 (CD11c/CD18).

 

Variants in this gene are the underlying cause for the autosomal recessive leukocyte adhesion deficiency type 1.