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HelpOrder Code LASF1 Lactic Acid, Spinal Fluid
Specimen Required
Specimen Type: Spinal fluid
Container/Tube: Sterile container
Specimen Volume: 1 mL
Collection Instructions: Centrifuge to remove any cellular material.
Useful For
Aid in differentiating between bacterial and viral meningitis
Aid in identifying increased anaerobic glycolysis or hypoxia associated with bacterial meningitis, cerebral infarction, cerebral arteriosclerosis, intracranial hemorrhage, hydrocephalus, traumatic brain injury, cerebral edema, epilepsy, and inborn errors of metabolism
Method Name
Colorimetric
Reporting Name
Lactic Acid, CSFSpecimen Type
CSFSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| CSF | Frozen (preferred) | 60 days | |
| Refrigerated | 24 hours | ||
| Ambient | 3 hours |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
Reference Values
0-2 days: 1.1-6.7 mmol/L
3-10 days: 1.1-4.4 mmol/L
11 days-17 years: 1.1-2.8 mmol/L
>17 years: 1.1-2.4 mmol/L
Method Description
Lactate concentration is determined using an enzymatic colorimetric method. L-lactate is oxidized to pyruvate by the specific enzyme lactate oxidase. Peroxidase is used to generate a colored dye using the hydrogen peroxide generated in the first reaction. The intensity of the color formed is directly proportional to the L-lactate concentration. It is determined by measuring the increase in absorbance.(Package insert: Roche Diagnostics Cobas 6000; LACT2 reagent package insert; Indianapolis, IN 46256. 02/2016)
Day(s) Performed
Monday through Sunday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
83605