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HelpOrder Code LBCS Labile Bound Copper, Serum
Ordering Guidance
If CUS1 / Copper, Serum is ordered with this test, it will be canceled, as copper testing will be automatically added as a part of this test order.
Specimen Required
Patient Preparation: High concentrations of gadolinium and iodine are known to potentially interfere with most inductively coupled plasma mass spectrometry-based metal tests. If either gadolinium- or iodine-containing contrast media has been administered, a specimen should not be collected for at least 96 hours.
Supplies:
-Metal Free Specimen Vial (T173)
-Metal Free B-D Tube (No Additive), 6 mL (T184)
Collection Container/Tube: 6-mL Plain, royal blue-top Vacutainer plastic trace element blood collection tube
Submission Container/Tube: 7-mL Metal-free, screw-capped, polypropylene vial
Specimen Volume: 0.75 mL serum
Collection Instructions:
1. For complete instructions, see Metals Analysis Specimen Collection and Transport.
2. Allow the specimen to clot for 30 minutes; then centrifuge the specimen to separate serum from the cellular fraction.
3. Remove the stopper. Carefully pour specimen into metal-free, polypropylene vial, avoiding transfer of the cellular components of blood. Do not insert a pipet into the serum to accomplish transfer, and do not ream the specimen with a wooden stick to assist with serum transfer.
4. Freeze sample on dry ice immediately after pouring off the serum.
Useful For
May be useful in the evaluation of copper-related disorders, including Wilson disease
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CUS1 | Copper, S | Yes | Yes |
Testing Algorithm
After a labile-bound copper serum result is obtained, a total copper serum test will be performed at an additional charge. The labile-bound copper concentration will be divided by the total copper concentration to calculate the labile-bound copper fraction result.
Special Instructions
Method Name
Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
Reporting Name
Labile Bound Copper, SSpecimen Type
SerumSpecimen Minimum Volume
Serum: 0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen | 28 days | METAL FREE |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | Reject |
Reference Values
Labile Bound Copper: <105 ng/mL
Labile Bound Copper Fraction:
Males: <10.5 %
Females: <8.1 %
Copper, Total:
0-2 months: 40-140 mcg/dL
3-6 months: 40-160 mcg/dL
7-9 months: 40-170 mcg/dL
10-12 months: 80-170 mcg/dL
13 months-10 years: 80-180 mcg/dL
11-17 years: 75-145 mcg/dL
Males:
≥18 years: 73-129 mcg/dL
Females:
≥18 years: 77-206 mcg/dL
Method Description
Total copper is analyzed by inductively coupled plasma mass spectrometry.(Unpublished Mayo method)
The labile bound copper fraction of serum is isolated from total copper through a series of filtration and chelation steps. Labile bound copper is then analyzed by inductively coupled plasma mass spectrometry.(Bitzer AC, Fox J, Day PL, et al. Establishment of a labile bound copper reference interval in a healthy population via an inductively coupled plasma mass spectrometry dual filtration-based assay. Arch Pathol Lab Med. 2024;148[7]:818-827. doi:10.5858/arpa.2023-0259-OA)
Day(s) Performed
Wednesday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82525