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Order Code LPS Lipase, Serum


Specimen Required


Patient Preparation: Patients should be fasting before the specimen is collected.

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Serum gel tube must be centrifuged within 2 hours of collection.

2. Red-top tube must be centrifuged, and the serum aliquoted into a plastic vial within 2 hours of collection.


Useful For

Investigating pancreatic disorders, usually pancreatitis

Method Name

Colorimetric Rate Reaction

Reporting Name

Lipase, S

Specimen Type

Serum

Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  365 days
  Ambient  7 days

Reject Due To

Gross hemolysis Reject
Other Collection tubes with glycerol-lubricated stoppers

Reference Values

13-60 U/L

Method Description

The lipase method is an enzymatic colorimetric method in which lipase catalyzes the hydrolysis of a natural 1,2-diglyceride to form monoglyceride and fatty acid. Monoglyceride is hydrolyzed by monoglyceride lipase to form glycerol and fatty acid. Glycerol is then phosphorylated by glycerol kinase in the presence of ATP to form glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase to form dihydroxyacetone phosphate and hydrogen peroxide. Subsequently, hydrogen peroxide reacts with 4-aminoamtipyrine and sodium N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine in the presence of peroxidase to form quinone diimine dye. The dye absorbs light at 550 nm. The rate of increase in absorbance at 550 nm is directly proportional to the pancreatic lipase activity in the specimen. The method is sensitive and specific for pancreatic lipase and utilizes co-lipase and deoxycholate as activators.(Package insert: Equal Diagnostics Lipase reagent, Exton, PA. V2.0 03/2019)

Day(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83690