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HelpOrder Code LPS Lipase, Serum
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Serum gel tube must be centrifuged within 2 hours of collection.
2. Red-top tube must be centrifuged, and the serum aliquoted into a plastic vial within 2 hours of collection.
Useful For
Investigating pancreatic disorders, usually pancreatitis
Method Name
Colorimetric
Reporting Name
Lipase, SSpecimen Type
SerumSpecimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 7 days |
| Frozen | 365 days | |
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Other | Collection tubes with glycerol-lubricated stoppers |
Reference Values
13-60 U/L
Method Description
Enzymatic colorimetric assay with 1,2-O-dilauryl-rac-glycero-3-glutaric-acid-(6-methylresorufin) ester as substrate. The chromogenic lipase substrate, 1,2-O-dilauryl-rac-glycero-3-glutaric-acid-(6-methylresorufin) ester is cleaved by the catalytic action of alkaline lipase solution to form 1,2-O-dilauryl-rac-glycerol and an unstable intermediate, glutaric acid-(6-methylresorufin) ester. This decomposes spontaneously in alkaline solution to form glutaric acid and methylresorufin. Addition of detergent and colipase increases the specificity of the assay for pancreatic lipase. The color intensity of the red dye formed is directly proportional to the lipase activity and can be determined photometrically.(Package insert: Lipase Colorimetric Assay. Roche Diagnostics; 09/2024)
Day(s) Performed
Monday through Sunday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
83690
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LPS | Lipase, S | 3040-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| LPS | Lipase, S | 3040-3 |