Home
HelpOrder Code MCRPL Macroprolactin, Serum
Additional Codes
| Mayo Test ID |
|---|
| MCRPL |
Reporting Name
Macroprolactin, SUseful For
Determining biologically active levels of prolactin, in asymptomatic patients with elevated prolactin levels
Ruling out the presence of macroprolactin
Specimen Type
SerumSpecimen Required
Patient Preparation: For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 7 days | |
| Frozen | 90 days | ||
| Ambient | 24 hours |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
Reference Values
PROLACTIN, TOTAL
Males
<18 years: not established
≥18 years: 4.0-15.2 ng/mL
Females
<18 years: not established
≥18 years: 4.8-23.3 ng/mL
PROLACTIN, UNPRECIPITATED
Males
<18 years: not established
≥18 years: 2.7-13.1 ng/mL
Females
<18 years: not established
≥18 years: 3.4-18.5 ng/mL
When the percent of the precipitated (complexed) prolactin fraction of the total prolactin is 60% or less, the result is considered negative for macroprolactin.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Day(s) Performed
Monday through Sunday
Method Name
TOPRL: Electrochemiluminescent Immunoassay
PEGPR: Polyethylene Glycol (PEG) Precipitation Followed by Electrochemiluminescent Immunoassay
CPT Code Information
84146 x 2
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TOPRL | Prolactin,Total,S | Yes, (order PRL) | Yes |
| PEGPR | Prolactin,Unprecipitated,S | No | Yes |
Method Description
TOPRL:
The Roche cobas e immunoassay Prolactin II method employs 2 monoclonal antibodies specifically directed against prolactin. A biotinylated monoclonal antibody and a second monoclonal antibody labeled with a ruthenium complex react with prolactin in the sample to form a sandwich complex. After the addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin. Application of a voltage to the electrode then induces chemiluminescent emission, which is measured.(Package insert: Elecsys Prolactin II, V 8.0, Roche 2017)
PEGPR:
Macroprolactin and oligomers can be precipitated by using a 25 % aqueous PEG solution (ratio 1+1). After centrifugation, the supernatant containing monomeric prolactin is used in the Elecsys Prolactin II assay in the same way as a native sample. The dilution effect which occurs during sample pretreatment and the coprecipitation of monomeric prolactin must be taken into consideration. Precipitated prolactin is calculated by subtracting the unprecipitated prolactin value from the total prolactin. The percentage of precipitated prolactin to total prolactin indicates the presence or absence of macroprolactin.(Package insert: Elecsys Prolactin II, V 8.0, Roche 2017)
Forms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.