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HelpOrder Code MDNS Methadone and Metabolites, Serum
Useful For
Compliance monitoring of methadone
Assessment of methadone toxicity
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Methadone and Metabolite, SSpecimen Type
Serum RedSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (Serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.0 mL
Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days |
| Frozen | 28 days | |
| Ambient | 14 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
Methadone:
Therapeutic: 100-400 ng/mL
Toxic: >2000 ng/mL
EDDP:
Not established
Cutoff concentrations by liquid chromatography tandem mass spectrometry:
Methadone: 10 ng/mL
EDDP: 5.0 ng/mL
Method Description
Liquid/liquid extraction of the serum sample followed by liquid chromatography tandem mass spectrometry.(Unpublished Mayo method)
Day(s) Performed
Tuesday, Thursday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
80299
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.