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Order Code MICF Antimicrobial Susceptibility Panel, Yeast, Varies

Infectious

Additional Codes

Mayo Test ID
MMLYP

Reporting Name

Susceptibility Panel, Yeast

Useful For

Determining in vitro quantitative antifungal susceptibility (minimum inhibitory concentration) of non-fastidious yeast

 

As an aid in the management of certain circumstances, such as:

-Refractory oropharyngeal infections due to Candida species in patients who appear to be experiencing therapeutic failure with standard agents at standard doses

-Invasive infections due to Candida species when the utility of azole antifungal agents is uncertain (eg, when the infection is due to a non-Candida albicans organism)

Specimen Type

Varies


Shipping Instructions


1. See Infectious Specimen Shipping Guidelines

2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.



Necessary Information


Specimen source and suspected organism identification are required. If identification of organism is not provided, FUNID / Culture Referred for Identification, Fungus, Varies will be performed at an additional charge.



Specimen Required


Specimen Type: Organism in pure culture

Supplies: Infectious Container, Large (T146)

Container/Tube: Sabouraud's dextrose agar slant

Specimen Volume: Infecting yeast isolate

Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)
  Refrigerated 

Reject Due To

Agar plate Reject

Reference Values

Results reported in mcg/mL

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Day(s) Performed

Monday through Saturday

Method Name

Minimum Inhibitory Concentration (MIC)

CPT Code Information

87186

Additional Tests

Test ID Reporting Name Available Separately Always Performed
SYP Susceptibility Yeast Panel No, (Bill Only) Yes

Testing Algorithm

When this test is ordered, the susceptibility yeast panel will be performed at an additional charge. All yeast submitted will have susceptibility testing performed and billed as appropriate.

Method Description

Yeast susceptibility is determined by colorimetric microdilution broth method using TREK Sensititre YeastOne Y011 Susceptibility Plate. Each plate is setup with appropriate dilutions of antifungal agents and a colorimetric indicator. After inoculation with a standardized suspension of organism in inoculum medium and incubation at 35° C for 24 to 48 hours; the minimum inhibitory concentrations for the test organism are determined by observing the lowest antifungal concentration showing inhibition of growth as evidenced by no color change of the test medium.(Clinical and Laboratory Standards Institute [CLSI]: Performance Standards for Antifungal Susceptibility Testing of Yeasts. 2nd ed. CLSI supplement M60. CLSI; 2020; CLSI: Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. 4th ed. CLSI standard M27. CLSI; 2017)

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.