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HelpOrder Code MMAP Methylmalonic Acid, Quantitative, Plasma
Reporting Name
Methylmalonic Acid, QN, PUseful For
Evaluating children with signs and symptoms of methylmalonic acidemia using plasma specimens
Evaluating individuals with signs and symptoms associated with a variety of causes of vitamin B12 (cobalamin) deficiency
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
PlasmaSpecimen Required
Collection Container/Tube:
Preferred: Green top (sodium heparin)
Acceptable: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions: Centrifuge and aliquot plasma into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma | Refrigerated (preferred) | 48 days |
| Ambient | 48 days | |
| Frozen | 48 days |
Reference Values
≤0.40 nmol/mL
Day(s) Performed
Monday through Friday
CPT Code Information
83921
Method Description
Methylmalonic acid (MMA) is determined by liquid chromatography tandem mass spectrometry (LC-MS/MS) stable isotope dilution analysis. The specimen is mixed with an internal standard (methyl-d3-malonic acid). MMA and d3-MMA are isolated by solid phase extraction. LC-MS/MS is performed using mobile phases and a short column (C18, 50 mm x 4.6 mm, 5 microns) to separate MMA and d3-MMA from the bulk of the specimen matrix. The MS/MS is operated in the multiple reaction monitoring (MRM) negative mode to follow the precursor to product species transitions. Separation of MMA/d3-MMA from the more physiologically abundant succinic acid is accomplished by the careful selection of MRM transitions and optimization of the LC separation. The ratios of the extracted peak areas of MMA to d3-MMA determined by LC-MS/MS are used to calculate the concentration of MMA present in the sample.(Unpublished Mayo method)
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Benign Hematology Test Request (T755)