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Order Code MMGEN Mycoplasma genitalium, Transcription-Mediated Amplification, Post-Prostatic Massage Fluid/Urine or Peritoneal Fluids


Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Post-prostatic massage fluid/urine (VBIII)

Supplies: Aptima Urine Transport Tube (T582)

Container/Tube: Aptima Urine Specimen Transport Tube

Specimen Volume: 15 to 20 mL

Collection Instructions:

1. Patient should not have urinated for at least 1 hour prior to specimen collection.

2. Patient should void a small amount of urine prior to prostatic massage. Pre-massage urine can be discarded.

3. Patient should cease voiding and a prostatic massage should be performed by urologist or other health care professional.

4. Collect post-massage urine into a sterile, plastic, preservative-free container.

5. Transfer 2 mL of urine into the Aptima Urine Specimen Transport Tube using the disposable pipette provided within 24 hours of collection. The correct volume of urine has been added when the fluid level is between the black fill lines on the urine transport tube.

6. Place the labels on the transport tube so the black fill lines are still visible for volume confirmation at Mayo Clinic Laboratories.

7. Transport and store post-prostatic massage fluid/urine (VBIII) specimen transport container at 2 to 30° C (refrigerate is preferred temperature) within 30 days of collection. Specimen cannot be sent frozen.

 

Specimen Type: Peritoneal fluid (pelvic wash, cul-de-sac fluid)

Supplies: Aptima Thin Prep Transport Tube (T652)

Container/Tube: Aptima Specimen Transfer Tube

Specimen Volume: 1 mL

Collection Instructions:

1. Collect specimen by paracentesis using needle and syringe. Refer to the references below.

2. Thoroughly vortex specimen upon receipt to the laboratory.

3. Transfer 1 mL of specimen into the Aptima Specimen Transfer kit using a disposable transfer pipette or a pipette tip containing a filter (aerosol barrier or hydrophobic plug) within 24 hours of collection.

4. Recap the specimen transfer tube tightly.

5. Transport and store specimen container at 2 to 30° C (refrigerate is preferred temperature) within 30 days of collection. Specimen cannot be sent frozen.


Useful For

Detecting Mycoplasma genitalium in cases of suspected infection in peritoneal fluid or prostatic secretion (VBIII) fluid/urine

 

This test is not intended for use in medico-legal applications.

Method Name

Transcription-Mediated Amplification

Reporting Name

Mycoplasma genitalium, TMA, Misc

Specimen Type

Varies

Specimen Minimum Volume

Peritoneal fluid: 1 mL; Post-prostatic massage fluid/urine (VBIII): See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Varies APTIMA VIAL

Reject Due To

Overfilled or underfilled urine transport tubes
Specimen collected into a SurePath Prep device or ThinPrep vial
Midstream/clean catch urine specimen
Transport tubes contain swabs
Reject

Reference Values

Negative

Method Description

The HOLOGIC APTIMA Combo 2 Assay combines the technologies of target capture, transcription-mediated amplification, and dual kinetic assay. The detection of the ribosomal RNA amplification product sequences (amplicon) is achieved using nucleic acid hybridization. Single-stranded chemiluminescent DNA probes are labeled and combined with amplicon to form stable RNA:DNA hybrids. Light emitted from the labeled RNA:DNA hybrids is measured as photon signals in a luminometer.(Package insert: APTIMA Mycoplasma genitalium Assay. AW-17946 Hologic, Inc; Rev 003, 03/2022)

Day(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87563

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.