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HelpOrder Code MMLNS Antimicrobial Susceptibility, Nocardia species and other Aerobic Actinomycetes, Varies
Infectious
Additional Codes
| Mayo Test ID |
|---|
| MMLNS |
Reporting Name
Susc, Aerobic ActinomycetesUseful For
Determining the resistance of species of Nocardia and other aerobic actinomycetes to antimicrobial agents.
This test is not useful for determining resistance of aerobic actinomycetes species of the following genera: Actinoallomurus, Actinocatenispora, Actinoplanes, Aeromicrobium, Croceifilum, Hazenella, Intrasporangium, Kineosphaera, Kitasatospora, Kribella, Kutzneria, Laceyella, Marinactinospora, Microbispora, Micromonospora, Nocardioides (not Nocardia), Phycicoccus, Piscicoccus, Prauserella, Risungbinella, Saccharothrix, Sphaerimonospora, Spirillospora, Streptosporangium, Terracoccus, or Thermoactinomyces.
Specimen Type
VariesOrdering Guidance
This test is intended for assessing antimicrobial susceptibility of Nocardia species and other pathogenic aerobic actinomycetes which include the more commonly isolated genera of Rhodococcus, Streptomyces, Gordonia, Dietzia, Tsukamurella, and Williamsia species, as well as the lesser isolated genera which include Actinomadura, Amycolatopsis, Dermatophilus, Kroppenstedtia, Nocardiopsis, Nonomuraea, Pseudonocardia, Saccharomonospora, and Saccharopolyspora species.
Certain genera of aerobic actinomycetes will not be accepted for susceptibility testing. Aerobic actinomycetes genera that will not be tested include the following: Actinoallomurus, Actinocatenispora, Actinoplanes, Aeromicrobium, Croceifilum, Hazenella, Intrasporangium, Kineosphaera, Kitasatospora, Kribella, Kutzneria, Laceyella, Marinactinospora, Microbispora, Micromonospora, Nocardioides (not Nocardia), Phycicoccus, Piscicoccus, Prauserella, Risungbinella, Saccharothrix, Sphaerimonospora, Spirillospora, Streptosporangium, Terracoccus, and Thermoactinomyces.
Additional Testing Requirements
If organism identification is not provided, CTB / Mycobacteria and Nocardia Culture, Varies or CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies must also be ordered and will be charged separately.
Shipping Instructions
1. For shipping information see Infectious Specimen Shipping Guidelines.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.
Necessary Information
1. Specimen source is required.
2. Organism identification is required unless either CTB / Mycobacteria and Nocardia Culture, Varies or CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies is also ordered.
Specimen Required
Specimen Type: Organism in pure culture
Supplies: Infectious Container, Large (T146)
Container/Tube:
Preferred: Middlebrook 7H10 agar slant without antimicrobials
Acceptable: Sabouraud's dextrose agar slant or similar media without antimicrobials (eg, 7H11 agar slant, LJ, MGIT [7H9] broth media)
Specimen Volume: Isolate
Collection Instructions: Organism must be in pure culture, actively growing.
Specimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Agar plate | Reject |
Reference Values
Interpretive criteria and reporting guidelines are followed using the Clinical Laboratory Standards Institute (CLSI) M24S document.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Day(s) Performed
Monday through Sunday
Method Name
Minimum Inhibitory Concentration (MIC)
CPT Code Information
87186
Special Instructions
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SSNS | Susceptibility Nocardia species | No, (Bill Only) | Yes |
Testing Algorithm
When this test is ordered, susceptibility for Nocardia species will be performed at an additional charge.
When the organism identification is Rhodococcus equi the additional reflex test will be performed at an additional charge for the additional antimicrobials vancomycin and rifampin.
Method Description
A standardized inoculum made from a pure culture of aerobic actinomycete is prepared and inoculated into wells of a microtiter plate containing different concentrations of antimicrobial agents.
Growth at specified breakpoints is indicative of resistance.(Clinical and Laboratory Standards Institute (CLSI). Susceptibility Testing of Mycobacteria, Nocardia spp., and Other Aerobic Actinomycetes. CLSI standard M24. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Susceptibility Testing of Mycobacteria, Nocardia spp., and Other Aerobic Actinomycetes. CLSI supplement M24S)
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MIC | Susceptibility, MIC | No, (Bill Only) | No |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.