Home
HelpOrder Code MPLVS MPL Exon 10 Mutation Detection, Varies
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Peripheral blood
Container/Tube: Lavender top (EDTA) or yellow top (ACD)
Specimen Volume: 3 mL
Collections Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube. Do not aliquot.
3. Label specimen as blood.
Specimen Stability: Ambient (preferred)/Refrigerate
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA) or yellow top (ACD)
Specimen Volume: 2 mL
Collections Instructions:
1. Invert several times to mix bone marrow.
2. Send specimens in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Stability: Ambient (preferred)/Refrigerate
Specimen Type: Extracted DNA from blood or bone marrow
Container/Tube: 1.5- to 2- mL tube
Specimen Volume: Entire specimen
Collection Instructions: Label specimen as extracted DNA from blood or bone marrow and provide indication of volume and concentration of DNA.
Specimen Stability Information: Frozen (preferred)/Refrigerated/Ambient
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Useful For
Aiding in the distinction between a reactive cytosis and a myeloproliferative neoplasm
Testing Algorithm
For more information see:
-Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation
-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation
Special Instructions
Method Name
Sanger Sequencing
Reporting Name
MPL Exon 10 Mutation Detection, VSpecimen Type
VariesSpecimen Minimum Volume
Blood, Bone marrow: 0.5 mL; Extracted DNA: 50 mcL at 20 ng/mcL concentration
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Bone marrow biopsies Slides paraffin shavings Moderately to severely clotted |
Reject |
Reference Values
An interpretive report will be provided.
Method Description
Genomic DNA is extracted from the blood or bone marrow sample, and the MPL exon 10 amplified using standard polymerase chain reaction. The entire exon 10 sequence is obtained using Sanger sequencing with analysis on an automated genetic analyzer.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81339-MPL (myeloproliferative leukemia virus oncogene, thrombopoietin receptor, TPOR) (eg, myeloproliferative disorder), exon 10 sequence