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Order Code MYOUR Myoglobin, Random, Urine

Important Note

If specimen is at ambient temperature, aliquot the urine to a urine myoglobin transport tube within 1 hour of collection. Refrigerate specimen.

 

If specimen is refrigerated, aliquot the urine to a urine myoglobin transport tube within 2 hours of collection.  Refrigerate specimen

Additional Codes

MAYO CODE:  MYGLU

Reporting Name

Myoglobin, Random, U

Useful For

Confirming the presence of a myopathy

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Urine


Specimen Required


Supplies: Urine Myoglobin Transport Tube (T691)

Container/Tube: Plastic, 5-mL urine myoglobin transport tube

Specimen Volume: 4 mL

Collection Instructions:

1. Collect a preservative-free, random urine specimen.

2. If specimen is at ambient temperature, aliquot the urine to a urine myoglobin transport tube within 1 hour of collection. Refrigerate specimen.

3. If specimen is at refrigerate temperature, aliquot the urine to a urine myoglobin transport tube within 2 hours of collection.

Additional Information: Urinary myoglobin is highly unstable unless alkalinized with sodium carbonate preservative.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated 7 days MYOGLOBIN TRANSPORT TUBE

Reference Values

0-24 mcg/L

Reference values have not been established for patients younger than 18 years; however myoglobin is not expected to be detectable in urine.

Day(s) Performed

Monday through Sunday

CPT Code Information

83874

Method Description

This myoglobin test is a sandwich-principal assay. The first incubation is 9 mcL of sample, a biotinylated monoclonal myoglobin-specific antibody, and a monoclonal myoglobin-specific antibody labeled with a ruthenium complex, which react to form a sandwich complex. In the second incubation, the complex becomes bound to the solid phase via interaction of biotin and streptavidin after addition of streptavidin-coated microparticles. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Results are determined via a calibration curve, which is instrument specifically generated by 2-point calibration, and a master curve provided via the cobas link.(Package insert: Elecsys Myoglobin. Roche Diagnostics; 10/2022)

Reject Due To

Use of any transport tube other than urine myoglobin transport tube Reject

Method Name

Electrochemiluminescent Immunoassay (ECLIA)