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HelpOrder Code NARC Narcolepsy-Associated Antigen, HLA-DQB1 Typing, Blood
Reporting Name
Narcolepsy Associated Ag, BUseful For
Ruling out a diagnosis of narcolepsy
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole Blood ACD-BSpecimen Required
Container/Tube:
Preferred: Yellow top (ACD solution B)
Acceptable: Yellow top (ACD solution A), lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions: Send whole blood specimen in original vial. Do not aliquot.
Additional Information: Specimen acceptability is based on extracted DNA concentration and not sample age.
Specimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood ACD-B | Refrigerated (preferred) | ||
| Ambient | |||
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
CPT Code Information
81376-HLA Class II typing, low resolution (eg, antigen equivalents); one locus (eg, HLA-DRB1/3/4/5, -DQB1, -DQA1, -DPB1, or -DPA1), each
Method Description
This assay applies Luminex technology to the reverse sequence specific oligonucleotide DNA typing method. First, target DNA is polymerase chain reaction (PCR)-amplified using a group-specific primer. The PCR product is biotinylated, which allows it to be detected using r-phycoerythrin-conjugated streptavidin. The PCR product is denatured and allowed to rehybridize to complementary DNA probes conjugated to fluorescently coded microspheres. A flow analyzer identifies the fluorescent intensity of phycoerythrin on each microsphere. The HLA class II allele or allele groups of the sample are determined by the positive and negative bead identified using a computer software program. The assignment of the human leukocyte antigen (HLA) typing is based on the reaction pattern compared to patterns associated with published HLA gene sequences.(Package insert: LABType SSO Typing Tests. One Lambda; Version 04, 11/11/2019)
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Method Name
Polymerase Chain Reaction (PCR)/Sequence-Specific Oligonucleotide Probes (SSO)