Sign in →

Order Code NMH1D N-Methylhistamine, 24 Hour, Urine


Ordering Guidance


Random urine collection is preferred for patients with episodic symptoms, eg, in the context of allergic reactions, brought on by specific environmental factors; order NMHR / N-Methylhistamine, Random, Urine.



Specimen Required


Only orderable as part of a profile. For more information see NMH24 / N-Methylhistamine, 24 Hour, Urine.

 

Patient Preparation: Patient must not be taking monoamine oxidase inhibitors (MAOIs) or aminoguanidine as these medications increase N-methylhistamine (NMH) levels.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Container/Tube: Plastic, 5-mL tube

Specimen Volume: 5 mL

Collection Instructions:

1. Collect urine for 24 hours.

2. No preservative.

3. Aliquot into plastic tube and send at refrigerate temperature.


Useful For

Screening for and monitoring of mastocytosis and disorders of systemic mast-cell activation, such as anaphylaxis and other forms of severe systemic allergic reactions as a part of a profile

 

Monitoring therapeutic progress in conditions that are associated with secondary, localized, low-grade persistent, mast-cell proliferation and activation such as interstitial cystitis

Method Name

Only orderable as part of a profile. For more information see NMH24 / N-Methylhistamine, 24 Hour, Urine.

 

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

N-Methylhistamine, 24 Hr, U

Specimen Type

Urine

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

Only orderable as part of a profile. For more information see NMH24 / N-Methylhistamine, 24 Hour, Urine.

 

0-5 years: 120-510 mcg/g creatinine

6-16 years: 70-330 mcg/g creatinine

>16 years: 30-200 mcg/g creatinine

Method Description

N-methylhistamine (NMH) is removed from urine using solid phase extraction. The eluate is analyzed using liquid chromatography/tandem mass spectrometry and quantified using a stable isotope labeled internal standard, d3-NMH. NMH and d3-NMH are detected in multiple reaction monitoring mode using the specific transitions for m/z 126 to m/z 109, and m/z 129 to m/z 112, respectively.(Unpublished Mayo method)

Day(s) Performed

Monday, Wednesday, Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

82542

Specimen Minimum Volume

3 mL