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Order Code OLIGS Oligoclonal Banding, Serum


Specimen Required


Only orderable as part of a profile. For more information see:

-OLIG / Oligoclonal Banding, Serum and Spinal Fluid

-MSP3 / Multiple Sclerosis (MS) Profile, Serum and Spinal Fluid

 

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Diagnosis of multiple sclerosis; especially useful in patients with equivocal clinical presentation and radiological findings

 

Determining number of serum oligoclonal bands in order to calculate the number of cerebrospinal fluid-specific bands present

Method Name

Only orderable as part of a profile. For more information see:

-OLIG / Oligoclonal Banding, Serum and Spinal Fluid

-MSP3 / Multiple Sclerosis (MS) Profile, Serum and Spinal Fluid

 

Isoelectric Focusing (IEF) with IgG Immunoblot Detection

Reporting Name

Serum Bands

Specimen Type

Serum

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Ambient  14 days
  Frozen  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia Reject
Gross icterus    OK

Reference Values

Only orderable as part of a profile. For more information see:

-OLIG / Oligoclonal Banding, Serum and Spinal Fluid

-MSP3 / Multiple Sclerosis (MS) Profile, Serum and Spinal Fluid

Method Description

The oligoclonal band (OCB) assay requires paired cerebrospinal fluid (CSF) and serum samples. Unconcentrated CSF and diluted serum are electrophoresed by isoelectric focusing. The separated immunoglobulins (Ig) are visualized by an IgG immunoblot, and OCBs that are present in the CSF and not in the serum are reported. The assay uses reagents from Helena Laboratories.(Keir G, Luxton RW, Thompson EJ: Isoelectric focusing of cerebrospinal fluid immunoglobulins G: an annotated update. Ann Clin Biochem. 1990;27:436-443; Hortin GL: Amino Acids, Peptides, and Proteins. In: Burtis CA, Bruns DE, eds. Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics. 7th ed. Elsevier; 2015:chap 18)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83916

Specimen Minimum Volume

0.4 mL