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HelpOrder Code OPTU Orthostatic Protein, Timed Collection, Urine
Reporting Name
Orthostatic Protein, UUseful For
Diagnosis of orthostatic proteinuria
As a second-order test for additional characterization of proteinuria of less than 3 grams/24 hours, particularly in children or adolescents
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| DOPTU | Daytime Orthostatic Protein, U | No | Yes |
| NOPTU | Nighttime Orthostatic Protein, U | No | Yes |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
UrineOrdering Guidance
This collection process requires 2 separate urine collections within a 24-hour period.
Collect specimen per instructions in Orthostatic Protein Measurement 24-Hour Urine: Collection Site Instructions (T546) in Special Instructions.
Necessary Information
This collection process requires 2 separate urine collections within a 24-hour period.
-Submit start and end times for collection and 16-hour volume (required).
-Submit start and end times for collection and 8-hour volume (required).
Specimen Required
Specimens should be collected before fluorescein is given or not collected until at least 24 hour later.
Supplies: 2 Sarstedt 5 mL Aliquot Tube (T914)
Daytime Collection
Container/Tube: Plastic, 5-mL tube
Specimen Volume: 4 mL
Collection Instructions:
1. Collect a 16-hour (daytime) urine specimen.
2. No preservative.
3. Invert well before taking 4-mL aliquot.
4. Do not over fill aliquot tube 4 mL at most.
5. Collect specimen per instructions in Orthostatic Protein Measurement 24-Hour Urine: Collection Site Instructions (T546) in Special Instructions.
Nighttime (Supine) Collection
Container/Tube: Plastic, 5-mL tube
Specimen Volume: 4 mL
Collection Instructions:
1. Collect an 8-hour (nighttime) urine specimen.
2. No preservative.
3. Invert well before taking 4-mL aliquot at most.
Specimen Minimum Volume
1 mL from 16-hour (daytime) urine collection/1 mL from 8-hour (nighttime) urine collection
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Urine | Refrigerated (preferred) | 14 days | |
| Frozen | 30 days | ||
| Ambient | 24 hours |
Special Instructions
Reference Values
Nighttime (supine) collection: <68 mg/8 hours
Reference values have not been established for patients <18 years of age.
Daytime collection: <197 mg/16 hours
Reference values have not been established for patients <18 years of age
CPT Code Information
84156 x 2
Method Description
The sample is preincubated in an alkaline solution containing EDTA, which denatures the protein and eliminates interference from magnesium ions. Benzethonium chloride is then added, producing turbidity.(Package insert: Total Protein Urine/CSF. Roche Diagnostics; V13.0 11/2018)
Reject Due To
| All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. |
Method Name
Turbidimetry
Forms
1. Orthostatic Protein Measurement 24-Hour Urine: Patient Collection Instructions in Special Instructions
2. If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.
Day(s) Performed
Monday through Sunday